Activator‐independent formation of a closed complex between σ54‐holoenzyme and nifH and nifU promoters of Klebsiella pneumoniae

M Buck, W Cannon - Molecular microbiology, 1992 - Wiley Online Library
The alterNatlve sigma factor σ54 is required for transcription of nitrogen fixation genes in
Klebsiella pneutnoniae and other diazotrophs. The nif genes, and other Eσ54‐dependent …

Control of nitrogen fixation genes in Klebsiella pneumoniae.

TR Hoover - 2000 - cabidigitallibrary.org
Expression of the nitrogen fixation (nif) genes in Klebsiella pneumoniae is tightly controlled
in response to oxygen and fixed nitrogen through a sophisticated hierarchy of regulatory …

In vitro studies of the domains of the nitrogen fixation regulatory protein NIFA

DK Berger, F Narberhaus, HS Lee… - Journal of bacteriology, 1995 - Am Soc Microbiol
The prokaryotic enhancer-binding protein NIFA is a multidomain transcriptional activator that
catalyzes the formation of open complexes at nitrogen fixation (nif) promoters by a …

Role of integration host factor in stimulating transcription from the σ54-dependent nifH promoter

E Santero, TR Hoover, AK North, DK Berger… - Journal of molecular …, 1992 - Elsevier
In a wide variety of nitrogen-fixing organisms among the Purple Bacteria (large division of
Gram-negative bacteria) the nitrogen fixation (nif) operons are transcribed by an alternative …

The isolated catalytic domain of NIFA, a bacterial enhancer-binding protein, activates transcription in vitro: activation is inhibited by NIFL.

DK Berger, F Narberhaus… - Proceedings of the …, 1994 - National Acad Sciences
The NIFA protein of Klebsiella pneumoniae is required for transcription of all nif (nitrogen
fixation) operons except the regulatory nifLA operon itself. NIFA activates transcription of nif …

Purification and in vitro activities of the native nitrogen fixation control proteins NifA and NifL

S Austin, M Buck, W Cannon, T Eydmann… - Journal of …, 1994 - Am Soc Microbiol
The prokaryotic enhancer-binding protein NifA stimulates transcription at a distance by
binding to sequences upstream of nitrogen fixation (nif) promoters and catalyzing the …

Activity of purified NIFA, a transcriptional activator of nitrogen fixation genes.

HS Lee, DK Berger, S Kustu - Proceedings of the National …, 1993 - National Acad Sciences
The NIFA protein activates transcription of nitrogen fixation (nif) operons by the sigma 54-
holoenzyme form of RNA polymerase. We purified active NIFA from Klebsiella pneumoniae …

Organization and function of binding sites for the transcriptional activator NifA in the Klebsiella pneumoniae nifE and nifU promoters

W Cannon, W Charlton, M Buck - Journal of molecular biology, 1991 - Elsevier
The interaction of the Klebsiella pneumoniae NifA protein, a σ 54-dependent activator, with
the nifE and nifU promoters was analysed. At these promoters NifA established contacts in …

Transcriptional activation of the nitrogenase promoter in vitro: adenosine nucleotides are required for inhibition of NIFA activity by NIFL

T Eydmann, E Söderbäck, T Jones, S Hill… - Journal of …, 1995 - Am Soc Microbiol
The enhancer-binding protein NIFA is required for transcriptional activation of nif promoters
by the alternative holoenzyme form of RNA polymerase, which contains the sigma factor …

The function of isolated domains and chimaeric proteins constructed from the transcriptional activators NifA and NtrC of Klebsiella pneumoniae

MH Drummond, A Contreras… - Molecular …, 1990 - Wiley Online Library
A model for the domain structure of σ54‐dependent transcriptional activators, based on
sequence data, has been tested by examining the function of truncated and chimaeric …