Bifunctional plant defence enzymes with chitinase and ribosome inactivating activities from Trichosanthes kirilowii cell cultures

NR Shih, KA McDonald, AP Jackman, T Girbés… - Plant Science, 1997 - Elsevier
Three bifunctional plant enzymes (named TKC 15, TKC 28-I, and TKC 28-II) with both
chitinase and RIP activity were purified from the medium of Trichosanthes kirilowii plant cell …

Isolation and characterization of new isoforms of trichosanthin from Trichosanthes kirilowii

P Narayanan, NK Mak, PB Luong, RNS Wong - Plant Science, 2002 - Elsevier
Three ribosome-inactivating proteins (RIPs) were isolated from the previously characterized
'trichosanthin'fraction prepared from the root tuber of Trichosanthes kirilowii. They were …

Co-expression of a modified maize ribosome-inactivating protein and a rice basic chitinase gene in transgenic rice plants confers enhanced resistance to sheath blight

JK Kim, IC Jang, R Wu, WN Zuo, RS Boston… - Transgenic …, 2003 - Springer
Abstract Chitinases, β-1, 3-glucanases, and ribosome-inactivating proteins are reported to
have antifungal activity in plants. With the aim of producing fungus-resistant transgenic …

Expression of a gene encoding trichosanthin in transgenic rice plants enhances resistance to fungus blast disease

H Yuan, X Ming, L Wang, P Hu, C An, Z Chen - Plant cell reports, 2002 - Springer
The gene encoding mature trichosanthin, a type I ribosome-inactivating protein isolated from
the tuber of Trichosanthes kirilowii Maximowicz, was transformed into calli of rice (Oryza …

Expression of a ribosome inhibiting protein (RIP) or a bacterial chitinase leads to fungal resistance in transgenic plants

J Logemann, G Jach, S Logemann, R Leah… - Mechanisms of plant …, 1993 - Springer
In order to protect plants against fungal attack, two genes (RIP, ChiA) encoding proteins with
in vitro antifungal activity were expressed in transgenic tobacco plants. 1. A barley derived …

Isolation and characterization of four type-1 ribosome-inactivating proteins, with polynucleotide:adenosine glycosidase activity, from leaves of Phytolacca dioica L.

A Di Maro, P Valbonesi, A Bolognesi, F Stirpe… - Planta, 1999 - Springer
Four type-1 (single-chain) ribosome-inactivating proteins (RIPs), with isoelectric points
between 9.5 and 9.7, were isolated from leaves of Phytolacca dioica L. The purification …

Increased accumulation of trichosanthin in Trichosanthes kirilowii induced by microorganisms

RNS Wong, NK Mak, WT Choi… - Journal of experimental …, 1995 - academic.oup.com
Trichosanthin, a type 1 ribosome-inactivating protein, is highly expressed in the root tuber of
Trichosanthes kirilowii when grown under normal greenhouse conditions. The expression …

Defense-related gene expression and enzyme activities in transgenic cotton plants expressing an endochitinase gene from Trichoderma virens in response to …

V Kumar, V Parkhi, CM Kenerley, KS Rathore - Planta, 2009 - Springer
There are many reports on obtaining disease-resistance trait in plants by overexpressing
genes from diverse organisms that encode chitinolytic enzymes. Current study represents an …

Biosynthesis of defense-related proteins in transformed root cultures of Trichosanthes kirilowii Maxim. var japonicum (Kitam.)

BJ Savary, HE Flores - Plant physiology, 1994 - academic.oup.com
We have established transformed (“hairy”) root cultures from Trichosanthes kirilowii Maxim.
var japonicum Kitam.(Cucurbitaceae) and four related species to study the biosynthesis of …

Chitinases and plant protection

R Broglie, K Broglie - Mechanisms of plant defense responses, 1993 - Springer
Recent studies suggest that the production of enzymes capable of degrading the cell walls
of invading phytopathogenic fungi is an important component of the defense response of …