[PDF][PDF] Exposure to DNA is insufficient for in vitro transgenesis of live bovine sperm and embryos
Transgenic mammals have been produced using sperm as vectors for exogenous DNA
(sperm-mediated gene transfer (SMGT)) in combination with artificial insemination. Our …
(sperm-mediated gene transfer (SMGT)) in combination with artificial insemination. Our …
Association of rabbit sperm cells with exogenous DNA
HJ Wang, AX Lin, YF Chen - Animal Biotechnology, 2003 - Taylor & Francis
The ability of rabbit spermatozoa to bind exogenous DNA during sperm‐mediated gene
transfer (SMGT) was tested in our study. Fresh collected semen, or fully capacitated sperm …
transfer (SMGT) was tested in our study. Fresh collected semen, or fully capacitated sperm …
Sperm-mediated gene transfer
M Lavitrano, M Busnelli, MG Cerrito… - Reproduction, fertility …, 2005 - CSIRO Publishing
Since 1989, a new method for the production of transgenic animals has been available,
namely sperm-mediated gene transfer (SMGT), based on the intrinsic ability of sperm cells to …
namely sperm-mediated gene transfer (SMGT), based on the intrinsic ability of sperm cells to …
Spontaneous uptake of exogenous DNA by goat spermatozoa and selection of donor bucks for sperm-mediated gene transfer
Y Zhao, M Yu, L Wang, Y Li, J Fan, Q Yang… - Molecular biology …, 2012 - Springer
Sperm-mediated gene transfer (SMGT) has been long heralded as a faster and cheaper
alternative to more commonly used methods of producing transgenic animals. In this study …
alternative to more commonly used methods of producing transgenic animals. In this study …
Spontaneous uptake of exogenous DNA by bull spermatozoa
M Anzar, MM Buhr - Theriogenology, 2006 - Elsevier
Sperm-mediated DNA transfer can be used to transfer exogenous DNA into the oocyte for
the production of transgenic animals. In spite of controversy in the literature, sperm-mediated …
the production of transgenic animals. In spite of controversy in the literature, sperm-mediated …
Sperm status and DNA dose play key roles in sperm/ICSI‐mediated gene transfer in caprine
F Shadanloo, MH Najafi, SM Hosseini… - Molecular …, 2010 - Wiley Online Library
In relation to the growing recent interest in the establishment of sperm‐mediated gene
transfer (SMGT) technology as a convenient and effective method for the simple production …
transfer (SMGT) technology as a convenient and effective method for the simple production …
Sperm mediated gene transfer in pig: Selection of donor boars and optimization of DNA uptake
Transgenic animals are produced primarily by microinjecting exogenous DNA into the male
pronuclei of a zygote. Microinjection is successful in mice but not efficient in farm animals …
pronuclei of a zygote. Microinjection is successful in mice but not efficient in farm animals …
[HTML][HTML] Exogenous DNA uptake by bovine spermatozoa does not induce DNA fragmentation
Sperm-mediated gene transfer (SMGT) is a fast and low-cost method used to produce
transgenic animals. The objective of this study was to evaluate the effects of the …
transgenic animals. The objective of this study was to evaluate the effects of the …
Sperm-mediated gene transfer
AWS Chan, CM Luetjens, GP Schatten - Current Topics in Developmental …, 2000 - Elsevier
Publisher Summary The first transgenic mouse was created in the early 1990s via
incubation of spermatozoa with exogenous DNA followed by in vitro fertilization (IVF). The …
incubation of spermatozoa with exogenous DNA followed by in vitro fertilization (IVF). The …
Transgene transmission in chickens by sperm-mediated gene transfer after seminal plasma removal and exogenous DNA treated with dimethylsulfoxide or N,N …
T Collares, VF Campos, PMM De Leon… - Journal of …, 2011 - Springer
Transgenic animals have been successfully produced by mass gene transfer techniques
such as sperm-mediated gene transfer (SMGT). The aim of this work was to demonstrate …
such as sperm-mediated gene transfer (SMGT). The aim of this work was to demonstrate …