Objective To evaluate the value of nanopore targeted sequencing in diagnosing pneumonia
pathogens. Methods This large-scale multicentre prospective study performed in 8 hospitals …

The gold standard for confirming bacterial infections is culture-positive, which has a long
sample-to-result turnaround time and poor sensitivity for unculturable and fastidious …

Background Next-generation sequencing of the metagenome (mNGS) is increasingly used
in pathogen diagnosis for infectious diseases due to its short detection time. The time for …

Background Diversified etiology of lower respiratory tract infection renders diagnosis
challenging. The mainstay microbial culture is time-consuming and constrained by variable …

At present, metagenomic next-generation sequencing (mNGS) based on Illumina platform
has been widely reported for pathogen detection. There are few studies on the diagnosis of …

Background Aetiology detection is crucial in the diagnosis and treatment of ventilator-
associated pneumonia (VAP). However, the detection method needs improvement. In this …

Objective To evaluate the efficacy of targeted nanopore sequencing technology for the
detection of Mycobacterium tuberculosis (M. tb.) in bronchoalveolar lavage fluid (BALF) …

Background Nanopore metagenomics has been used for infectious disease diagnosis for
bacterial pathogens. However, this technology currently lacks comprehensive performance …

Background Metagenomic sequencing of respiratory microbial communities for pathogen
identification in pneumonia may help overcome the limitations of culture-based methods. We …

Respiratory tract infection (RTI) remains a significant cause of morbidity and mortality across
the globe. The optimal management of RTI relies upon timely pathogen identification via …