Effector‐induced self‐association and conformational changes in the enhancer‐binding protein NTRC
ME Farez‐Vidal, TJ Wilson, BE Davidson… - Molecular …, 1996 - Wiley Online Library
The Klebsiella pneumoniae nitrogen regulatory protein NTRC is a response regulator which
activates transcription in response to nitrogen limitation, and is a member of the family of σN …
activates transcription in response to nitrogen limitation, and is a member of the family of σN …
Substitutions at a single amino acid residue in the nitrogen‐regulated activator protein NTRC differentially influence its activity in response to phosphorylation
R Dixon, T Eydmann, N Henderson… - Molecular …, 1991 - Wiley Online Library
Four substitutions at serine residue 160 which increase the activity of the σ54‐dependent
activator protein NTRC in the absence of NTRB have been analysed in detail. Mutagenesis …
activator protein NTRC in the absence of NTRB have been analysed in detail. Mutagenesis …
The function of isolated domains and chimaeric proteins constructed from the transcriptional activators NifA and NtrC of Klebsiella pneumoniae
MH Drummond, A Contreras… - Molecular …, 1990 - Wiley Online Library
A model for the domain structure of σ54‐dependent transcriptional activators, based on
sequence data, has been tested by examining the function of truncated and chimaeric …
sequence data, has been tested by examining the function of truncated and chimaeric …
Mechanism of activation of a response regulator: interaction of NtrC-P dimers induces ATPase activity
I Mettke, U Fiedler, V Weiss - Journal of bacteriology, 1995 - Am Soc Microbiol
NtrC is the transcriptional activator for nitrogen-regulated promoters and, as a response
regulator, belongs to the protein family of two-component systems. The activity of all …
regulator, belongs to the protein family of two-component systems. The activity of all …
Structural and functional analyses of activating amino acid substitutions in the receiver domain of NtrC: evidence for an activating surface
The bacterial enhancer-binding protein NtrC activates transcription when phosphorylated on
aspartate 54 in its amino (N)-terminal regulatory domain or when altered by constitutively …
aspartate 54 in its amino (N)-terminal regulatory domain or when altered by constitutively …
Phosphorylation-induced signal propagation in the response regulator NtrC
The bacterial enhancer-binding protein NtrC is a well-studied response regulator in a two-
component regulatory system. The amino (N)-terminal receiver domain of NtrC modulates …
component regulatory system. The amino (N)-terminal receiver domain of NtrC modulates …
Phosphorylation-independent dimer-dimer interactions by the enhancer-binding activator NtrC of Escherichia coli: a third function for the C-terminal domain
The response regulator NtrC transcriptionally activates genes of the nitrogen-regulated (Ntr)
response. Phosphorylation of its N-terminal receiver domain stimulates an essential …
response. Phosphorylation of its N-terminal receiver domain stimulates an essential …
Interaction of purified NtrC protein with nitrogen regulated promoters from Klebsiella pneumoniae
The product of the Klebsiella pneumoniae nitrogen regulatory gene ntrC has been purified
and shown to be a dimeric protein of subunit molecular weight 54Kd, designated NtrC. In an …
and shown to be a dimeric protein of subunit molecular weight 54Kd, designated NtrC. In an …
The bacterial enhancer-binding protein NtrC as a molecular machine
I Rombel, A North, I Hwang… - Cold Spring Harbor …, 1998 - symposium.cshlp.org
METHODS Overproduction and purification of proteins and assays of their activities. Proteins
were overexpressed and purified as described previously (Porter et al. 1993; Flashner et al …
were overexpressed and purified as described previously (Porter et al. 1993; Flashner et al …
Influence of a mutation in the putative nucleotide binding site of the nitrogen regulatory protein NTRC on its positive control function
S Austin, C Kundrot, R Dixon - Nucleic acids research, 1991 - academic.oup.com
A mutation, serine 170 to alanine, in the proposed ATP binding site of the activator protein
NTRC prevents transcriptional activation at σ 54-dependent promoters both in vivo and in …
NTRC prevents transcriptional activation at σ 54-dependent promoters both in vivo and in …