Transcription control engineering and applications in synthetic biology
MD Engstrom, BF Pfleger - Synthetic and systems biotechnology, 2017 - Elsevier
In synthetic biology, researchers assemble biological components in new ways to produce
systems with practical applications. One of these practical applications is control of the flow …
systems with practical applications. One of these practical applications is control of the flow …
Promoter engineering: recent advances in controlling transcription at the most fundamental level
Synthetic control of gene expression is critical for metabolic engineering efforts. Specifically,
precise control of key pathway enzymes (heterologous or native) can help maximize product …
precise control of key pathway enzymes (heterologous or native) can help maximize product …
Synthetic biology: tools to design microbes for the production of chemicals and fuels
The engineering of biological systems to achieve specific purposes requires design tools
that function in a predictable and quantitative manner. Recent advances in the field of …
that function in a predictable and quantitative manner. Recent advances in the field of …
Contemporary tools for regulating gene expression in bacteria
Insights from novel mechanistic paradigms in gene expression control have led to the
development of new gene expression systems for bioproduction, control, and sensing …
development of new gene expression systems for bioproduction, control, and sensing …
Tunable promoters in synthetic and systems biology
T Dehli, C Solem, PR Jensen - Reprogramming microbial metabolic …, 2012 - Springer
Synthetic and systems biologists need standardized, modular and orthogonal tools yielding
predictable functions in vivo. In systems biology such tools are needed to quantitatively …
predictable functions in vivo. In systems biology such tools are needed to quantitatively …
Advances and current limitations in transcript-level control of gene expression
JM Leavitt, HS Alper - Current opinion in biotechnology, 2015 - Elsevier
Highlights•Transcriptional engineering provides tight control of gene expression in
eukaryotes.•Synthetic promoters provide iterative improvements in strength.•Orthogonal …
eukaryotes.•Synthetic promoters provide iterative improvements in strength.•Orthogonal …
Recent advances in mammalian synthetic biology—design of synthetic transgene control networks
M Tigges, M Fussenegger - Current opinion in biotechnology, 2009 - Elsevier
Capitalizing on an era of functional genomic research, systems biology offers a systematic
quantitative analysis of existing biological systems thereby providing the molecular inventory …
quantitative analysis of existing biological systems thereby providing the molecular inventory …
Expression optimization and synthetic gene networks in cell-free systems
Synthetic biology offers great promise to a variety of applications through the forward
engineering of biological function. Most efforts in this field have focused on employing living …
engineering of biological function. Most efforts in this field have focused on employing living …
Diversity, versatility and complexity of bacterial gene regulation mechanisms: opportunities and drawbacks for applications in synthetic biology
I Bervoets, D Charlier - FEMS microbiology reviews, 2019 - academic.oup.com
Gene expression occurs in two essential steps: transcription and translation. In bacteria, the
two processes are tightly coupled in time and space, and highly regulated. Tight regulation …
two processes are tightly coupled in time and space, and highly regulated. Tight regulation …
Post-translational tools expand the scope of synthetic biology
EJ Olson, JJ Tabor - Current opinion in chemical biology, 2012 - Elsevier
Synthetic biology is improving our understanding of and ability to control living organisms.
To date, most progress has been made by engineering gene expression. However …
To date, most progress has been made by engineering gene expression. However …