Efficient yeast surface-display of novel complex synthetic cellulosomes

H Tang, J Wang, S Wang, Y Shen, D Petranovic… - Microbial Cell …, 2018 - Springer
Background The self-assembly of cellulosomes on the surface of yeast is a promising
strategy for consolidated bioprocessing to convert cellulose into ethanol in one step. Results …

Surface display of a functional minicellulosome by intracellular complementation using a synthetic yeast consortium and its application to cellulose hydrolysis and …

SL Tsai, G Goyal, W Chen - Applied and Environmental …, 2010 - Am Soc Microbiol
In this paper, we report the surface assembly of a functional minicellulosome by using a
synthetic yeast consortium. The basic design of the consortium consisted of four different …

Self-surface assembly of cellulosomes with two miniscaffoldins on Saccharomyces cerevisiae for cellulosic ethanol production

LH Fan, ZJ Zhang, XY Yu, YX Xue… - Proceedings of the …, 2012 - National Acad Sciences
Yeast to directly convert cellulose and, especially, the microcrystalline cellulose into
bioethanol, was engineered through display of minicellulosomes on the cell surface of …

In vitro assembly of minicellulosomes with two scaffoldins on the yeast cell surface for cellulose saccharification and bioethanol production

LH Fan, ZJ Zhang, XY Yu, YX Xue, MM Wang… - Process …, 2013 - Elsevier
The present paper reports in vitro strategies for assembly of minicellulosomes with two
miniscaffoldins on the Saccharomyces cerevisiae cell surface. It was carried out through …

Functional display of complex cellulosomes on the yeast surface via adaptive assembly

SL Tsai, NA DaSilva, W Chen - ACS synthetic biology, 2013 - ACS Publications
A new adaptive strategy was developed for the ex vivo assembly of a functional tetravalent
designer cellulosome on the yeast cell surface. The design is based on the use of (1) a …

Yeast surface display of trifunctional minicellulosomes for simultaneous saccharification and fermentation of cellulose to ethanol

F Wen, J Sun, H Zhao - Applied and environmental microbiology, 2010 - Am Soc Microbiol
By combining cellulase production, cellulose hydrolysis, and sugar fermentation into a
single step, consolidated bioprocessing (CBP) represents a promising technology for biofuel …

Engineered pentafunctional minicellulosome for simultaneous saccharification and ethanol fermentation in Saccharomyces cerevisiae

Y Liang, T Si, EL Ang, H Zhao - Applied and environmental …, 2014 - Am Soc Microbiol
Several yeast strains have been engineered to express different cellulases to achieve
simultaneous saccharification and fermentation of lignocellulosic materials. However …

Constructing a yeast to express the largest cellulosome complex on the cell surface

M Anandharaj, YJ Lin, RP Rani… - Proceedings of the …, 2020 - National Acad Sciences
Cellulosomes, which are multienzyme complexes from anaerobic bacteria, are considered
nature's finest cellulolytic machinery. Thus, constructing a cellulosome in an industrial yeast …

Functional Assembly of Minicellulosomes on the Saccharomyces cerevisiae Cell Surface for Cellulose Hydrolysis and Ethanol Production

SL Tsai, J Oh, S Singh, R Chen… - Applied and …, 2009 - Am Soc Microbiol
We demonstrated the functional display of a miniscaffoldin on the Saccharomyces
cerevisiae cell surface consisting of three divergent cohesin domains from Clostridium …

Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase

S Kim, SH Baek, K Lee, JS Hahn - Microbial Cell Factories, 2013 - Springer
Background Cellulosic biomass is considered as a promising alternative to fossil fuels, but
its recalcitrant nature and high cost of cellulase are the major obstacles to utilize this …