Mammalian genomes contain active recombinase recognition sites

B Thyagarajan, MJ Guimaraes, AC Groth, MP Calos - Gene, 2000 - Elsevier
Recombinases derived from microorganisms mediate efficient site-specific recombination.
For example, the Cre recombinase from bacteriophage P1 efficiently carries out …

Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase.

B Sauer, N Henderson - The New Biologist, 1990 - europepmc.org
Cre is a 38-kD protein from bacteriophage P1 that catalyzes site-specific recombination
between 34-bp loxP sequences. Our previous work has shown that Cre can perform site …

Directed evolution of the site specificity of Cre recombinase

SW Santoro, PG Schultz - Proceedings of the National …, 2002 - National Acad Sciences
Cre recombinase from bacteriophage P1 recognizes a 34-bp recombination site, loxP, with
exquisite sequence specificity and catalyzes the site-specific insertion, excision, or …

[HTML][HTML] Using an in vivo phagemid system to identify non-compatible loxP sequences

RW Siegel, R Jain, A Bradbury - FEBS letters, 2001 - Elsevier
The site-specific recombination system of bacteriophage P1 is composed of the Cre
recombinase that recognizes a 34-bp loxP site. The Cre/loxP system has been extensively …

A genetic screen identifies novel non‐compatible loxP sites

SJ Langer, AP Ghafoori, M Byrd… - Nucleic acids …, 2002 - academic.oup.com
The ability of the Cre/lox system to make precise genomic modifications is a tremendous
accomplishment. However, recombination between cis‐linked heterospecific lox sites limits …

Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1.

B Sauer, N Henderson - Proceedings of the National …, 1988 - National Acad Sciences
The Cre protein encoded by the coliphage P1 is a 38-kDa protein that efficiently promotes
both intra-and intermolecular synapsis and recombination of DNA both in Escherichia coli …

Cryptic loxP sites in mammalian genomes: genome-wide distribution and relevance for the efficiency of BAC/PAC recombineering techniques

S Semprini, TJ Troup, N Kotelevtseva… - Nucleic acids …, 2007 - academic.oup.com
Cre is widely used for DNA tailoring and, in combination with recombineering techniques, to
modify BAC/PAC sequences for generating transgenic animals. However, mammalian …

[PDF][PDF] Cre recombinase: the universal reagent for genome tailoring

A Nagy - genesis, 2000 - academia.edu
In September 1998 the workshop for “Conditional Genome Alterations” in Cold Spring
Harbor had a simple but important take-home message:“Cre works”(Rossant and McMahon …

Production and characterization of human 293 cell lines expressing the site-specific recombinase Cre

L Chen, M Anton, FL Graham - Somatic cell and molecular genetics, 1996 - Springer
We have constructed 293 cell lines expressing the site-specific Cre recombinase from
bacteriophage P1, that acts on a 34 bp target sequence called loxP. Stably transformed cells …

VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering

E Suzuki, M Nakayama - Nucleic acids research, 2011 - academic.oup.com
We developed two new site-specific recombination systems named VCre/VloxP and
SCre/SloxP for genome engineering. Their recognition sites are different from Cre …