Central domain of the positive control protein NifA and its role in transcriptional activation
W Cannon, M Buck - Journal of molecular biology, 1992 - Elsevier
The positive control protein NifA of Klebsiella pneumoniae activates transcription by RNA
polymerase containing σ 54 by catalysing open promoter complex formation. We show that …
polymerase containing σ 54 by catalysing open promoter complex formation. We show that …
Organization and function of binding sites for the transcriptional activator NifA in the Klebsiella pneumoniae nifE and nifU promoters
W Cannon, W Charlton, M Buck - Journal of molecular biology, 1991 - Elsevier
The interaction of the Klebsiella pneumoniae NifA protein, a σ 54-dependent activator, with
the nifE and nifU promoters was analysed. At these promoters NifA established contacts in …
the nifE and nifU promoters was analysed. At these promoters NifA established contacts in …
The isolated catalytic domain of NIFA, a bacterial enhancer-binding protein, activates transcription in vitro: activation is inhibited by NIFL.
DK Berger, F Narberhaus… - Proceedings of the …, 1994 - National Acad Sciences
The NIFA protein of Klebsiella pneumoniae is required for transcription of all nif (nitrogen
fixation) operons except the regulatory nifLA operon itself. NIFA activates transcription of nif …
fixation) operons except the regulatory nifLA operon itself. NIFA activates transcription of nif …
Secondary structure and DNA binding by the C‐terminal domain of the transcriptional activator NifA from Klebsiella pneumoniae
P Ray, KJ Smith, RA Parslow, R Dixon… - Nucleic acids …, 2002 - academic.oup.com
Abstract The NifA protein of Klebsiella pneumoniae is required for transcriptional activation
of all nitrogen fixation (nif) operons except the regulatory nifLA genes. At these operons, NifA …
of all nitrogen fixation (nif) operons except the regulatory nifLA genes. At these operons, NifA …
Mutations in the RNA polymerase recognition sequence of the Klebsiella pneumoniae nifH promoter permitting transcriptional activation in the absence …
M Buck, W Cannon - Nucleic acids research, 1989 - academic.oup.com
Positive control of the wild-type Klebsiella pneumoniae nifH promoter by the NifA protein
requires that NifA is bound at the upstream activator sequence (UAS). By introducing base …
requires that NifA is bound at the upstream activator sequence (UAS). By introducing base …
The Klebsiella pneumoniae nifJ promoter: analysis of promoter elements regulating activation by the Nif A promoter
W Charlton, W Cannon, M Buck - Molecular microbiology, 1993 - Wiley Online Library
The nifJ and nifH promoters of Klebsiella pneumoniae are divergently transcribed σ; 54‐
dependent promoters that are positively activated by the NifA protein. NifA binds to upstream …
dependent promoters that are positively activated by the NifA protein. NifA binds to upstream …
In vitro studies of the domains of the nitrogen fixation regulatory protein NIFA
DK Berger, F Narberhaus, HS Lee… - Journal of bacteriology, 1995 - Am Soc Microbiol
The prokaryotic enhancer-binding protein NIFA is a multidomain transcriptional activator that
catalyzes the formation of open complexes at nitrogen fixation (nif) promoters by a …
catalyzes the formation of open complexes at nitrogen fixation (nif) promoters by a …
The C-terminal domain of NifL is sufficient to inhibit NifA activity
F Narberhaus, HS Lee, RA Schmitz, L He… - Journal of …, 1995 - Am Soc Microbiol
In Klebsiella pneumoniae, transcription of all nif (nitrogen fixation) operons except the
regulatory nifLA operon itself is regulated by the proteins NifA and NifL. NifA, an enhancer …
regulatory nifLA operon itself is regulated by the proteins NifA and NifL. NifA, an enhancer …
The function of isolated domains and chimaeric proteins constructed from the transcriptional activators NifA and NtrC of Klebsiella pneumoniae
MH Drummond, A Contreras… - Molecular …, 1990 - Wiley Online Library
A model for the domain structure of σ54‐dependent transcriptional activators, based on
sequence data, has been tested by examining the function of truncated and chimaeric …
sequence data, has been tested by examining the function of truncated and chimaeric …
Suppression analysis of positive control mutants of NifA reveals two overlapping promoters for Klebsiella pneumoniae rpoN
RA Grande, B Valderrama, E Morett - Journal of molecular biology, 1999 - Elsevier
Activation of gene expression relies on direct molecular interactions between the RNA
polymerase and transcription factors. Eubacterial enhancer-binding proteins (EBPs) activate …
polymerase and transcription factors. Eubacterial enhancer-binding proteins (EBPs) activate …