Recent advances in nanopore sequencing technology have led to a substantial increase in
throughput and sequence quality. Together, these improvements may permit real-time …

Background Diversified etiology of lower respiratory tract infection renders diagnosis
challenging. The mainstay microbial culture is time-consuming and constrained by variable …

Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene is a practical and reliable
measure for taxonomic profiling of bacterial communities. This chapter describes the …

Next-generation sequencing is rapidly finding footholds in numerous microbiological fields,
including infectious disease diagnostics. Here, we describe a molecular inversion probe …

Next-generation sequencing applications are increasingly used for detection and
characterization of antimicrobial-resistant pathogens in clinical settings. Oxford Nanopore …

The identification of microbial species has depended predominantly upon culture-based
techniques. However, the difficulty with which types of organisms are cultured implies that …

The convergence of several technologies such as ultrafast nanopore sequencing,
biosensors, the Internet of things (IoT), cloud computing, computational intelligence …

Urinary tract infections (UTIs) are among the most common acquired bacterial infections in
humans. The current gold standard method for identification of uropathogens in clinical …

Fast and accurate identification of pathogens is an essential task in healthcare settings.
Second-generation sequencing platforms such as Illumina have greatly expanded the …

US public health agencies have employed next-generation sequencing (NGS) as a tool to
quickly identify foodborne pathogens during outbreaks. Although established short-read …