[HTML][HTML] A framework for real-time monitoring, analysis and adaptive sampling of viral amplicon nanopore sequencing

R Munro, N Holmes, C Moore, M Carlile, A Payne… - Frontiers in …, 2023 - frontiersin.org
The ongoing SARS-CoV-2 pandemic demonstrates the utility of real-time sequence analysis
in monitoring and surveillance of pathogens. However, cost-effective sequencing requires …

ONTdeCIPHER: an amplicon-based nanopore sequencing pipeline for tracking pathogen variants

E Cherif, FS Thiam, M Salma, G Rivera-Ingraham… - …, 2022 - academic.oup.com
Motivation Amplicon-based nanopore sequencing is increasingly used for molecular
surveillance during epidemics (eg ZIKA, EBOLA) or pandemics (eg SARS-CoV-2). However …

[HTML][HTML] Rapid PCR-based nanopore adaptive sequencing improves sensitivity and timeliness of viral clinical detection and genome surveillance

Y Lin, Y Dai, Y Liu, Z Ren, H Guo, Z Li, J Li… - Frontiers in …, 2022 - frontiersin.org
Nanopore sequencing has been widely used for the real-time detection and surveillance of
pathogens with portable MinION. Nanopore adaptive sequencing can enrich on-target …

[HTML][HTML] poreCov-An Easy to Use, Fast, and Robust Workflow for SARS-CoV-2 Genome Reconstruction via Nanopore Sequencing

C Brandt, S Krautwurst, R Spott, M Lohde… - Frontiers in …, 2021 - frontiersin.org
In response to the SARS-CoV-2 pandemic, a highly increased sequencing effort has been
established worldwide to track and trace ongoing viral evolution. Technologies, such as …

Enrichment by hybridisation of long DNA fragments for Nanopore sequencing

SE Eckert, JZM Chan, D Houniet… - Microbial …, 2016 - microbiologyresearch.org
Enrichment of DNA by hybridisation is an important tool which enables users to gather target-
focused next-generation sequence data in an economical fashion. Current in-solution …

[HTML][HTML] Nanopore sequencing of SARS-CoV-2: Comparison of short and long PCR-tiling amplicon protocols

B Brejová, K Boršová, V Hodorová, V Čabanová… - PLoS …, 2021 - journals.plos.org
Surveillance of the SARS-CoV-2 variants including the quickly spreading mutants by rapid
and near real-time sequencing of the viral genome provides an important tool for effective …

InterARTIC: an interactive web application for whole-genome nanopore sequencing analysis of SARS-CoV-2 and other viruses

JM Ferguson, H Gamaarachchi, T Nguyen… - …, 2022 - academic.oup.com
Motivation InterARTIC is an interactive web application for the analysis of viral whole-
genome sequencing (WGS) data generated on Oxford Nanopore Technologies (ONT) …

[HTML][HTML] Real-time SARS-CoV-2 diagnostic and variants tracking over multiple candidates using nanopore DNA sequencing

F Stüder, JL Petit, S Engelen, MA Mendoza-Parra - Scientific reports, 2021 - nature.com
Since December 2019, a novel coronavirus responsible for a severe acute respiratory
syndrome (SARS-CoV-2) is accountable for a major pandemic situation. The emergence of …

Validating whole genome nanopore sequencing, using Usutu virus as an example

BBO Munnink, DF Nieuwenhuijse, RS Sikkema… - JoVE (Journal of …, 2020 - jove.com
Whole genome sequencing can be used to characterize and to trace viral outbreaks.
Nanopore-based whole genome sequencing protocols have been described for several …

Nanopore sequencing: a rapid solution for infectious disease epidemics

Y Cao, J Li, X Chu, H Liu, W Liu, D Liu - Science China Life Sciences, 2019 - Springer
Emerging and re-emerging infectious diseases have given rise to a large number of human
infections, morbidity, and heavy economic burden, including the Middle East respiratory …