Imaged capillary isoelectric focusing: applications in the pharmaceutical industry and recent innovations of the technology

J Wu, W McElroy, J Pawliszyn, CD Heger - TrAC Trends in Analytical …, 2022 - Elsevier
Capillary isoelectric focusing with whole-column imaging detection (icIEF) technology is now
the mainstream technology to perform analytical isoelectric focusing analysis in …

Coupling imaged capillary isoelectric focusing with mass spectrometry using a nanoliter valve

C Montealegre, C Neusüß - Electrophoresis, 2018 - Wiley Online Library
Imaged capillary isoelectric focusing (iCIEF) is a powerful separation technique applied
frequently for the analysis of biotherapeutics. However, direct mass spectrometric …

Capillary isoelectric focusing–the role of markers of isoelectric point and recent applications in the field

F Duša, A Kubesová, J Šalplachta… - TrAC Trends in Analytical …, 2023 - Elsevier
Capillary isoelectric focusing (cIEF) is a very powerful separation technique. However,
successful implementation of this technique in various fields of science requires a few …

Capillary isoelectric focusing with whole column imaging detection for analysis of proteins and peptides

Q Mao, J Pawliszyn - Journal of biochemical and biophysical methods, 1999 - Elsevier
Whole column imaging detection has been developed for capillary isoelectric focusing
(CIEF) of proteins and peptides. In this imaged CIEF technique, a solution of sample and …

A novel reagent significantly improved assay robustness in imaged capillary isoelectric focusing

X Zhang, S Voronov, N Mussa, Z Li - Analytical biochemistry, 2017 - Elsevier
Abstract Imaged Capillary Isoelectric Focusing (icIEF) has been used as primary method for
charge variants analysis of therapeutic antibodies and proteins [1],[9]. Proteins tend to …

Axially illuminated fluorescence imaging detection for capillary isoelectric focusing on Teflon capillary

T Huang, J Pawliszyn - Analyst, 2000 - pubs.rsc.org
An axially illuminated laser induced fluorescence (LIF) whole column imaging detection
(WCID) method was developed for capillary isoelectric focusing (CIEF). In this method, an …

Universal detection for capillary isoelectric focusing without mobilization using concentration gradient imaging system

J Wu, J Pawliszyn - Analytical Chemistry, 1992 - ACS Publications
Reagents. All chemicals and solution preparation procedures were the same as those used
in previous experiments (14). Samples used include-chymotrypsin (type II, Sigma) …

Capillary isoelectric focusing with whole column detection and a membrane sample preparation system

J Wu, C Tragas, A Watson, J Pawliszyn - Analytica chimica acta, 1999 - Elsevier
Progress of the research on capillary isoelectric focusing (cIEF)–whole column detection is
reviewed. Three kinds of whole column detectors have been developed for cIEF: universal …

Whole-column-imaging detection for capillary isoelectric focusing and capillary electrophoresis

X Wu, J Wu, J Pawliszyn - LC-GC North America, 2001 - go.gale.com
In this article, the authors review recent developments in the research of whole-column-
imaging detection for capillary electrophoresis (CE). Whole-column-imaging detection was …

Dynamics of capillary isoelectric focusing in the absence of fluid flow: High-resolution computer simulation and experimental validation with whole column optical …

Q Mao, J Pawliszyn, W Thormann - Analytical chemistry, 2000 - ACS Publications
A 150-component, dynamic electrophoresis simulator was developed and applied to the
description of capillary isoelectric focusing (CIEF) of amphoteric substances in quiescent …