In the course of studies on glutamine-dependent carbamyl phosphate synthetase from
Aerobacter aerogenes, we purified another protein which was found to be glutamate …

The amidotransferase family of enzymes utilizes the ammonia derived from the hydrolysis of
glutamine for a subsequent chemical reaction catalyzed by the same enzyme. The ammonia …

Publisher Summary This chapter discusses aminotransferases in higher plants. The
aminotransferases or transaminases are enzymes catalyzing the transfer of an amino group …

The Bacillus subtilis gene encoding glutamine phosphoribosylpyrophosphate
amidotransferase (amidophosphoribosyltransferase) was cloned in pBR322. This gene is …

MATERIALS AND METHODS Chemicals and Enzymes. Restriction enzymes were pur-
chased from either Promega, Stratagene, or United States Biochemical Corporation. All …

Ammonia-limited Aerobacter aerogenes, Erwinia carotovora, Pseudomonas fluorescens,
Bacillus subtilis and B. megaterium synthesized glutamate from NH3 and 2-oxoglutarate by …

Glutamine phosphoribosylpyrophosphate (PRPP) amidotransferase from Escherichia coli
exhibits a basal PRPP-independent glutaminase activity having ak cat/K m that is 0.3% of …

A family of four glutamine amidotransferases has a homologous glutamine amide transfer
domain, designated purF-type, that is named after purF-encoded glutamine …

The reaction catalyzed by ornithine carbamoyltransferase can participate in either the
anabolism or the catabolism of arginine. The carbamoylation of ornithine, yielding citrulline …

A purification procedure has been developed for the isolation of
phosphoribosylpyrophosphate amidotransferase (EC 2.4. 2.14, ribosylamine 5-phosphate …