High throughput sequencing of 16S ribosomal RNA gene amplicons is a cost‐effective
method for characterization of oral bacterial communities. However, before undertaking …

Background and objective The advent of next-generation sequencing has significantly
facilitated characterization of the oral microbiome. Despite great efforts in streamlining the …

Background Most studies examining the commensal human oral microbiome are focused on
disease or are limited in methodology. In order to diagnose and treat diseases at an early …

Culture-independent nucleic acid technologies have been extensively applied to the
analysis of oral bacterial communities associated with healthy and diseased conditions …

Culture-independent nucleic acid technologies have been extensively applied to the
analysis of oral bacterial communities associated with healthy and diseased conditions …

The profiling of bacterial communities by the sequencing of housekeeping genes such as
that encoding the small subunit ribosomal RNA has revealed the extensive diversity of …

Amplification and sequencing of 16S amplicons are widely used for profiling the structure of
oral microbiota. However, it remains not clear whether and to what degree DNA extraction …

Comparing bacterial 16S rDNA sequences to GenBank and other large public databases via
BLAST often provides results of little use for identification and taxonomic assignment of the …

There is currently no criterion to select appropriate bioinformatics tools and reference
databases for analysis of 16S rRNA amplicon data in the human oral microbiome. Our study …

To date, metagenomic studies have relied on the utilization and analysis of reads obtained
using 454 pyrosequencing to replace conventional Sanger sequencing. After extensively …