Efficient engineering of vascularized ectopic bone from human embryonic stem cell–derived mesenchymal stem cells

H Domev, M Amit, I Laevsky, A Dar… - … Engineering Part A, 2012 - liebertpub.com
Human mesenchymal stem cells (hMSCs) can be derived from various adult and fetal
tissues. However, the quality of tissues for the isolation of adult and fetal hMSCs is donor …

Establishment of an early vascular network promotes the formation of ectopic bone

RM Eman, HAW Meijer, FC Öner… - … Engineering Part A, 2016 - liebertpub.com
Vascularization is crucial for the induction of bone formation. In this study, we investigated
the application of two subtypes of peripheral blood-derived endothelial progenitor cells …

Engineering vascularized bone graft with osteogenic and angiogenic lineage differentiated bone marrow mesenchymal stem cells

R Zhang, Z Gao, W Geng, X Yan, F Chen… - Artificial organs, 2012 - Wiley Online Library
Tissue‐engineered bone provides a promising method for the rehabilitation of acquired
bone defects and congenital deformities. However, generating a vascular supply to the …

Engineering pre-vascularized bone-like tissue from human mesenchymal stem cells through simulating endochondral ossification

Z Lin, X Zhang, MR Fritch, Z Li, B Kuang, PG Alexander… - Biomaterials, 2022 - Elsevier
Currently, most in vitro engineered bone tissues do not contain viable blood vessel systems,
so the vascularization depends on post-implantation angiogenesis from the host, which is …

Effect of donor variation on osteogenesis and vasculogenesis in hydrogel cocultures

I Pennings, LA van Dijk, J van Huuksloot… - Journal of tissue …, 2019 - Wiley Online Library
To introduce a functional vascular network into tissue‐engineered bone equivalents, human
endothelial colony forming cells (ECFCs) and multipotent mesenchymal stromal cells …

Enhanced bone tissue regeneration of a biomimetic cellular scaffold with co‐cultured MSCs‐derived osteogenic and angiogenic cells

L Li, J Li, Q Zou, Y Zuo, B Cai, Y Li - Cell proliferation, 2019 - Wiley Online Library
Objectives The bone tissue engineering primarily focuses on three‐dimensional co‐culture
systems, which physical and biological properties resemble the cell matrix of actual tissues …

An in vitro bone tissue regeneration strategy combining chondrogenic and vascular priming enhances the mineralization potential of mesenchymal stem cells in vitro …

FE Freeman, MG Haugh… - Tissue Engineering Part A, 2015 - liebertpub.com
Chondrogenic priming (CP) of mesenchymal stem cells (MSCs) and coculture of MSCs with
human umbilical vein endothelial stem cells (HUVECs) both have been shown to …

Derivation of stromal (skeletal and mesenchymal) stem-like cells from human embryonic stem cells

A Mahmood, L Harkness, BM Abdallah… - Stem cells and …, 2012 - liebertpub.com
Derivation of bone forming cells (osteoblasts) from human embryonic stem cells (hESCs) is
a prerequisite for their use in clinical applications. However, there is no standard protocol for …

Superior osteogenic capacity for bone tissue engineering of fetal compared with perinatal and adult mesenchymal stem cells

ZY Zhang, SH Teoh, MSK Chong, JT Schantz… - Stem …, 2009 - academic.oup.com
Mesenchymal stem cells (MSCs) from human adult bone marrow (haMSCs) represent a
promising source for bone tissue engineering. However, their low frequencies and limited …

Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cells

RA Kopher, VR Penchev, MS Islam, KL Hill, S Khosla… - Bone, 2010 - Elsevier
Mesenchymal stem/stromal cells (MSCs) have been isolated from various tissues and
utilized for an expanding number of therapies. The developmental pathways involved in …