The broad host range plasmid RK2 has previously been found to contain three separate
regions of the genome involved in replication and maintenance in Escherichia coli (CM …

It has previously been concluded that regions tentatively designated trfA and trfB, located at
16–18.7 and 54–56 kb, respectively, on the genome of broad host range plasmid RK2 …

The sites of cleavage on the map of the broad-host-range plasmid RK2 (56 kilobases) were
determined for the BglII, PstI, and SmaI restriction enzymes, and the determinants for …

Replication of broad host-range plasmid RK2 depends on a cis-acting vegetative replication
origin oriV RK2 and the polypeptide product (s) of the trans-acting gene trfA as well as on …

Replication and incompatibility properties in Escherichia coli of DNA segments from the
replication origin region of plasmid RK2 have been investigated. A 393 bp Hpa II fragment …

Summary Figurski et al.(1982) have reported that certain loci on the broad host range
plasmid RK2 (kil functions) can be cloned only in the presence of other trans-acting …

Broad host-range plasmid RK2 is a member of the Escherichia coli incompatibility group P.
Unlike most other groups of plasmids, members of the P group are capable of efficient …

We have examined the relationship of kilB to the other known determinants which map in the
14′–22′ region of RK2. These are trfA, which encodes a diffusible replication function …

pRK212. 2, a derivative of the broad host range plasmid RK2, contains two Eco RI cleavage
fragments, A and B, neither of which can replicate by itself in Escherichia coli. Fragment A …

The broad-host-range plasmid RK2 and its derivatives are maintained in Gram-negative
bacteria at a specific copy number that appears to be determined by a series of direct …