Next generation sequencing (NGS) is challenged by structural and copy number variations
larger than the typical read length of several hundred bases. Third-generation sequencing …

Despite recent improvements in sequencing methods, there remains a need for assays that
provide high sequencing depth and comprehensive variant detection. Current methods …

Nanopore sequencing technology can rapidly and directly interrogate native DNA
molecules. Often we are interested only in interrogating specific areas at high depth, but …

Nanopore sequencers enable selective sequencing of single molecules in real time by
individually reversing the voltage across specific nanopores. Thus DNA molecules can be …

Nanopore sequencers can be used to selectively sequence certain DNA molecules in a pool
by reversing the voltage across individual nanopores to reject specific sequences, enabling …

The CRISPR-Cas9 system is widely used to permanently delete genomic regions via dual
guide RNAs. Genomic rearrangements induced by CRISPR-Cas9 can occur, but continuous …

High-throughput short-read sequencing has revolutionized how transcriptomes are
quantified and annotated. However, while Illumina short-read sequencers can be used to …

Background: The ability to obtain long read lengths during DNA sequencing has several
potentially important practical applications. Especially long read lengths have been reported …

A substantial fraction of the human genome is difficult to interrogate with short-read DNA
sequencing technologies due to paralogy, complex haplotype structures, or tandem repeats …

Third-generation sequencing can be used in human cancer genomics and epigenomic
research. Oxford Nanopore Technologies (ONT) recently released R10. 4 flow cell, which …