Bioconjugation of proteins with a paramagnetic NMR and fluorescent tag

F Huang, YY Pei, HH Zuo, JL Chen… - … –A European Journal, 2013 - Wiley Online Library
Site‐specific labeling of proteins with lanthanide ions offers great opportunities for
investigating the structure, function, and dynamics of proteins by virtue of the unique …

Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis

JL Chen, Y Zhao, YJ Gong, BB Pan, X Wang… - Journal of Biomolecular …, 2018 - Springer
Organic synthesis of a ligand with high binding affinities for paramagnetic lanthanide ions is
an effective way of generating paramagnetic effects on proteins. These paramagnetic effects …

Prospects for lanthanides in structural biology by NMR

G Otting - Journal of biomolecular NMR, 2008 - Springer
The advent of different lanthanide-binding reagents has made site-specific labelling of
proteins with paramagnetic lanthanides a viable proposition. This brings many powerful …

Noncovalent tagging proteins with paramagnetic lanthanide complexes for protein study

Z Wei, Y Yang, QF Li, F Huang… - … –A European Journal, 2013 - Wiley Online Library
The site‐specific labeling of proteins with paramagnetic lanthanides offers unique
opportunities for NMR spectroscopic analysis in structural biology. Herein, we report an …

Design, synthesis, and evaluation of a lanthanide chelating protein probe: CLaNP-5 yields predictable paramagnetic effects independent of environment

PHJ Keizers, A Saragliadis, Y Hiruma… - Journal of the …, 2008 - ACS Publications
Immobilized lanthanide ions offer the opportunity to refine structures of proteins and the
complexes they form by using restraints obtained from paramagnetic NMR experiments. We …

[HTML][HTML] Phosphoserine for the generation of lanthanide-binding sites on proteins for paramagnetic nuclear magnetic resonance spectroscopy

S Mekkattu Tharayil, MC Mahawaththa… - Magnetic …, 2021 - mr.copernicus.org
Pseudocontact shifts (PCSs) generated by paramagnetic lanthanide ions provide valuable
long-range structural information in nuclear magnetic resonance (NMR) spectroscopic …

Structural study of proteins by paramagnetic lanthanide probe methods

T Saio, F Inagaki - … Approaches of NMR Spectroscopy: Methodology and …, 2018 - Springer
Long-range structural information provided by the paramagnetic lanthanide probe methods
is invaluable in the structural analysis of proteins, particularly protein complexes and multi …

Two-point anchoring of a lanthanide-binding peptide to a target protein enhances the paramagnetic anisotropic effect

T Saio, K Ogura, M Yokochi, Y Kobashigawa… - Journal of biomolecular …, 2009 - Springer
Paramagnetic lanthanide ions fixed in a protein frame induce several paramagnetic effects
such as pseudo-contact shifts and residual dipolar couplings. These effects provide long …

Accelerating structural life science by paramagnetic lanthanide probe methods

T Saio, K Ishimori - Biochimica et Biophysica Acta (BBA)-General Subjects, 2020 - Elsevier
We describe the recent progress of structural analysis methods exploiting paramagnetic
lanthanide ions. In NMR spectroscopy, the paramagnetic effects induced by the trivalent …

The application of paramagnetic lanthanoid ions in NMR spectroscopy on proteins

WM Liu, M Overhand, M Ubbink - Coordination Chemistry Reviews, 2014 - Elsevier
Lanthanoids are gaining popularity as paramagnetic centers for high resolution nuclear
magnetic resonance (NMR) spectroscopy. They provide valuable angular and long-distance …