Exogenous DNA length and quantity affect the transfection rate, but not sperm viability during sperm-mediated gene transfer

WB Feitosa, MP Milazzotto, CM Mendes, AM da Rocha… - Gene Reports, 2022 - Elsevier
Spermatozoa have a spontaneous ability to take up exogenous DNA in a process regulated
by specific mechanisms. This ability has been used to carry exogenous DNA into oocytes …

Effect of exogenous DNA on bovine sperm functionality using the sperm mediated gene transfer (SMGT) technique

S Canovas, A Gutierrez‐Adan… - Molecular Reproduction …, 2010 - Wiley Online Library
Sperm mediated gene transfer (SMGT) could provide the opportunity to carry out
transgenesis on a mass scale using spermatozoa as vectors for exogenous DNA. However …

[PDF][PDF] A method for tracing exogenous DNA uptake in live spermatozoa and embryos

Z Liu, Y Zhao, X Zhang, Y Mu, J Wang… - Polish journal of …, 2018 - journals.pan.pl
Sperm-mediated gene transfer (SMGT) is a simple method for producing transgenic animals.
Due to the lack of repeatability in spermatozoa binding and internalization of exogenous …

[HTML][HTML] Exogenous DNA uptake by bovine spermatozoa does not induce DNA fragmentation

WB Feitosa, CM Mendes, MP Milazzotto, AM Rocha… - Theriogenology, 2010 - Elsevier
Sperm-mediated gene transfer (SMGT) is a fast and low-cost method used to produce
transgenic animals. The objective of this study was to evaluate the effects of the …

Effect of transfection and co-incubation of bovine sperm with exogenous DNA on sperm quality and functional parameters for its use in sperm-mediated gene transfer

ME Arias, E Sánchez-Villalba, A Delgado, R Felmer - Zygote, 2017 - cambridge.org
Sperm-mediated gene transfer (SMGT) is based on the capacity of sperm to bind exogenous
DNA and transfer it into the oocyte during fertilization. In bovines, the progress of this …

精子结合外源DNA 的特征及影响因素

叶华虎, 刘彦, 刘珠果, 宋维平, 卢建申, 周艳荣… - 遗传, 2006 - chinagene.cn
Exogenous DNA localization and the frequency of spermatozoa carrying exogenous DNA
after sperm/DNA co-culture are key to a successful sperm mediated-gene transfer (SMGT) …

Spontaneous uptake of exogenous DNA by goat spermatozoa and selection of donor bucks for sperm-mediated gene transfer

Y Zhao, M Yu, L Wang, Y Li, J Fan, Q Yang… - Molecular biology …, 2012 - Springer
Sperm-mediated gene transfer (SMGT) has been long heralded as a faster and cheaper
alternative to more commonly used methods of producing transgenic animals. In this study …

Cell viability of bovine spermatozoa subjected to DNA electroporation and DNAse I treatment

PV Cavalcanti, MP Milazzotto, R Simões, M Nichi… - Theriogenology, 2016 - Elsevier
Many mechanisms involved in sperm-mediated gene transfer (SMGT) are still unknown. It is
still a matter of debate whether exogenous DNA fragments incorporated by the embryo are …

[PDF][PDF] Effects of sperm parameters and incubation conditions of sperms with foreign DNA in ovine sperm transfection

KH Pajooh, P Tajik - 2020 - ivj.ir
Sperm-mediated gene transfer (SMGT) based on the intrinsic ability of sperms to bind and
take-up of exogenous DNA was introduced in 1989. From that time on, it has been a …

Association of rabbit sperm cells with exogenous DNA

HJ Wang, AX Lin, YF Chen - Animal Biotechnology, 2003 - Taylor & Francis
The ability of rabbit spermatozoa to bind exogenous DNA during sperm‐mediated gene
transfer (SMGT) was tested in our study. Fresh collected semen, or fully capacitated sperm …