In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae

PK Wong, D Popham, J Keener, S Kustu - Journal of bacteriology, 1987 - Am Soc Microbiol
In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K.
pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an …

Transcriptional activation of the Klebsiella pneumoniae nitrogenase promoter may involve DNA loop formation

M Buck, W Cannon, J Woodcock - Molecular microbiology, 1987 - Wiley Online Library
Transcriptional activation of nitrogen fixation genes by Nrt A in Klebsiella pneumoniae
requires an upstream Nif A binding site. We now report that the introduction of haif turns of …

Requirements for transcriptional activation in vitro of the nitrogen‐regulated gInA and nifLA promoters from Klebsiella pneumoniae: dependence on activator …

S Austin, N Henderson, R Dixon - Molecular microbiology, 1987 - Wiley Online Library
Three proteins involved in nitrogen regulation In Klebsiella pneumoniae, NTRA, NTRB and
NTRC, have been purified. In a defined in vitro system all three NTR proteins are required …

Deletion analysis of the Klebsiella pneumoniae nitrogenase promoter: importance of spacing between conserved sequences around positions-12 and-24 for …

M Buck - Journal of bacteriology, 1986 - Am Soc Microbiol
The nitrogen fixation promoters of Klebsiella pneumoniae are atypical procaryotic promoters
lacking the usual-10 and-35 elements, requiring instead conserved sequences around-12 …

Mutational analysis of the Klebsiella pneumoniae nitrogenase promoter: sequences essential for positive control by nifA and ntrC (glnG) products

DW Ow, Y Xiong, Q Gu, SC Shen - Journal of bacteriology, 1985 - Am Soc Microbiol
ntr (nitrogen regulated) and nif (nitrogen fixation) promoters are structurally similar to each
other but bear no resemblance to canonic Escherichia coli promoters. ntr promoters are …

Deletion analysis of the nitrogen fixation regulatory gene, nifL of Klebsiella pneumoniae

M Arnott, C Sidoti, S Hill, M Merrick - Archives of microbiology, 1989 - Springer
A number of in-frame deletions have been constructed in the Klebsiella pneumoniae
regulatory gene nifL. The effects of each nifL mutation on NifA-mediated expression from the …

Nitrogen control of the nif regulon in Klebsiella pneumoniae: involvement of the ntrA gene and analogies between ntrC and nifA.

MJ Merrick - The EMBO Journal, 1983 - embopress.org
The ntrC and nifA gene products of Klebsiella pneumoniae are transcriptional activators
involved in general nitrogen control and nif‐specific regulation, respectively. Multicopy …

Repressor properties of the nifL gene product in Klebsiella pneumoniae

M Merrick, S Hill, H Hennecke, M Hahn, R Dixon… - Molecular and General …, 1982 - Springer
Certain mutations in the nifL gene of the Klebsiella pneumoniae nitrogen fixation (nif) gene
cluster resulted in altered nif regulaiton such that nitrogenase synthesis was no longer …

Interaction of purified NtrC protein with nitrogen regulated promoters from Klebsiella pneumoniae

T Hawkes, M Merrick, R Dixon - Molecular and General Genetics MGG, 1985 - Springer
The product of the Klebsiella pneumoniae nitrogen regulatory gene ntrC has been purified
and shown to be a dimeric protein of subunit molecular weight 54Kd, designated NtrC. In an …

Mechanism of coordinated synthesis of the antagonistic regulatory proteins NifL and NifA of Klebsiella pneumoniae

F Govantes, JA Molina-Lopez, E Santero - Journal of bacteriology, 1996 - Am Soc Microbiol
The nifLA operon of Klebsiella pneumoniae codes for the two antagonistic regulatory
proteins which control expression of all other nitrogen fixation genes. NifA is a transcriptional …