A 1.4‐kb PstI–HpaI DNA fragment carrying the Klebsiella pneumoniae nifM gene has been
sequenced; nifM has been shown to encode a 30.6‐kDa polypeptide. Two other open …

Active Fe protein of nitrogenase was synthesised in a non‐nitrogen fixing organism when
Escherichia coli was transformed with a plasmid encoding only two nif‐specific genes, nifH …

Two hundred and thirty-five Nif-strains of Klebsiella pneumoniae were characterized by two-
dimensional polyacrylamide gel electrophoresis. Forty-two of these strains were tested …

NifQ-mutants of Klebsiella pneumoniae are defective in nitrogen fixation due to an elevated
requirement for molybdenum. When millimolar concentrations of molybdate were added to …

The nifA gene of Klebsiella pneumoniae, which encodes the transcriptional activator of nif
gene expression, was cloned into a number of plasmid vectors to obtain high-level synthesis …

The role of conserved nucleotides in nitrogen-fixation promoter function has been examined
using both oligonucleotide and chemical mutagenesis to introduce base changes in the …

We have determined the precise point of transcrip tion initiation for five nif (nitrogen fixation)
operons of Klebsiella pneumoniae and sequenced the promoters. Our results show that nifF …

The MoFe protein of nitrogenase from Klebsiella pneumoniae contains an iron‐molybdenum
cofactor, FeMoco, the synthesis or processing of which involves the products of at least five …

A series of plasmids encoding various Klebsiella pneumoniae nif (nitrogen fixation) genes
were constructed to determine which were required to produce active iron (Fe) protein in …

Complementation tests were performed with various nif mutations for delineating the nif
genes. The nif-/nif-heterogenotes were constructed by transferring nif plasmid mutant PRD …