Species-level resolution of 16S rRNA gene amplicons sequenced through the MinION™ portable nanopore sequencer

A Benítez-Páez, KJ Portune, Y Sanz - Gigascience, 2016 - academic.oup.com
Background The miniaturised and portable DNA sequencer MinION™ has been released to
the scientific community within the framework of an early access programme to evaluate its …

Multi-locus and long amplicon sequencing approach to study microbial diversity at species level using the MinION™ portable nanopore sequencer

A Benítez-Páez, Y Sanz - Gigascience, 2017 - academic.oup.com
The miniaturized and portable DNA sequencer MinION™ has demonstrated great potential
in different analyses such as genome-wide sequencing, pathogen outbreak detection and …

Evaluation of PCR conditions for characterizing bacterial communities with full-length 16S rRNA genes using a portable nanopore sequencer

S Fujiyoshi, A Muto-Fujita, F Maruyama - Scientific Reports, 2020 - nature.com
Abstract MinION (Oxford Nanopore Technologies), a portable nanopore sequencer, was
introduced in 2014 as a new DNA sequencing technology. MinION is now widely used …

Evaluation of Oxford Nanopore's MinION sequencing device for microbial whole genome sequencing applications

AD Tyler, L Mataseje, CJ Urfano, L Schmidt… - Scientific reports, 2018 - nature.com
Abstract The MinION sequencer (Oxford Nanopore Technologies) is a paradigm shifting
device allowing rapid, real time long read sequencing of nucleic acids. Yet external …

Benchmarking the MinION: evaluating long reads for microbial profiling

RM Leidenfrost, DC Pöther, U Jäckel, R Wünschiers - Scientific Reports, 2020 - nature.com
Nanopore based DNA-sequencing delivers long reads, thereby simplifying the
decipherment of bacterial communities. Since its commercial appearance, this technology …

Is Oxford Nanopore sequencing ready for analyzing complex microbiomes?

LJ Kerkhof - FEMS microbiology ecology, 2021 - academic.oup.com
This minireview will discuss the improvements in Oxford Nanopore (Oxford; sequencing
technology that make the MinION a viable platform for microbial ecology studies. Specific …

Bacterial and viral identification and differentiation by amplicon sequencing on the MinION nanopore sequencer

A Kilianski, JL Haas, EJ Corriveau, AT Liem… - …, 2015 - academic.oup.com
Abstract Background The MinION™ nanopore sequencer was recently released to a
community of alpha-testers for evaluation using a variety of sequencing applications. Recent …

INC-Seq: accurate single molecule reads using nanopore sequencing

C Li, KR Chng, EJH Boey, AHQ Ng, A Wilm… - …, 2016 - academic.oup.com
Background Nanopore sequencing provides a rapid, cheap and portable real-time
sequencing platform with the potential to revolutionize genomics. However, several …

Nanopore is preferable over Illumina for 16S amplicon sequencing of the gut microbiota when species-level taxonomic classification, accurate estimation of richness …

M Szoboszlay, L Schramm, D Pinzauti, J Scerri… - Microorganisms, 2023 - mdpi.com
Nanopore sequencing is a promising technology used for 16S rRNA gene amplicon
sequencing as it can provide full-length 16S reads and has a low up-front cost that allows …

The newest Oxford Nanopore R10. 4.1 full-length 16S rRNA sequencing enables the accurate resolution of species-level microbial community profiling

T Zhang, H Li, S Ma, J Cao, H Liao… - Applied and …, 2023 - Am Soc Microbiol
The long-read amplicon provides a species-level solution for the community. With the
improvement of nanopore flowcells, the accuracy of Oxford Nanopore Technologies (ONT) …