Recent technical advancements have facilitated the mapping of epigenomes at single-cell
resolution; however, the throughput and quality of these methods have limited their …

Chromatin accessibility, a crucial component of genome regulation, has primarily been
studied in homogeneous and simple systems, such as isolated cell populations or early …

Profiling multiple omic layers in a single cell enables the discovery and analysis of biological
phenomena that are not apparent from analysis of mono‐omic data. While methods for …

Recent advancements in next-generation sequencing technologies and accompanying
reductions in cost have led to an explosion of techniques to examine DNA accessibility and …

ATAC-seq is a widely-applied assay used to measure genome-wide chromatin accessibility;
however, its ability to detect active regulatory regions can depend on the depth of …

Most genetic variations associated with human complex traits are located in non-coding
genomic regions. Therefore, understanding the genotype-to-phenotype axis requires a …

Programs of gene transcription are controlled by cis-acting DNA elements, including
enhancers, silencers, and promoters. Local accessibility of chromatin has proven to be a …

Background Single-cell RNA-sequencing (scRNA-seq) measures gene expression in single
cells, while single-nucleus ATAC-sequencing (snATAC-seq) quantifies chromatin …

Chromatin immunoprecipitation, DNase I hypersensitivity and transposase-accessibility
assays combined with high-throughput sequencing enable the genome-wide study of …

This unit describes Assay for Transposase‐Accessible Chromatin with high‐throughput
sequencing (ATAC‐seq), a method for mapping chromatin accessibility genome‐wide. This …