Nanopore sequencing resolves elusive long tandem-repeat regions in mitochondrial genomes

L Kinkar, RB Gasser, BL Webster, D Rollinson… - International Journal of …, 2021 - mdpi.com
Long non-coding, tandem-repetitive regions in mitochondrial (mt) genomes of many
metazoans have been notoriously difficult to characterise accurately using conventional …

Approaches to whole mitochondrial genome sequencing on the Oxford Nanopore MinION

RR Zascavage, CL Hall, K Thorson… - Current Protocols in …, 2019 - Wiley Online Library
Traditional approaches for interrogating the mitochondrial genome often involve laborious
extraction and enrichment protocols followed by Sanger sequencing. Although preparation …

Long PCR amplification of the entire mitochondrial genome from individual helminths for direct sequencing

M Hu, AR Jex, BE Campbell, RB Gasser - Nature Protocols, 2007 - nature.com
Exploring mitochondrial (mt) genomes has significant implications for various fundamental
research areas, including mt biochemistry and physiology, and, importantly, such genomes …

Toward next-generation sequencing of mitochondrial genomes—focus on parasitic worms of animals and biotechnological implications

AR Jex, DTJ Littlewood, RB Gasser - Biotechnology advances, 2010 - Elsevier
Helminths (worms) include parasitic nematodes (roundworms) and platyhelminths
(flatworms). These worms are abundant, and many of them are of agricultural, aquacultural …

First record of a tandem-repeat region within the mitochondrial genome of Clonorchis sinensis using a long-read sequencing approach

L Kinkar, ND Young, WM Sohn… - PLOS Neglected …, 2020 - journals.plos.org
Background Mitochondrial genomes provide useful genetic markers for systematic and
population genetic studies of parasitic helminths. Although many such genome sequences …

A novel fragmented mitochondrial genome in the protist pathogen Toxoplasma gondii and related tissue coccidia

S Namasivayam, RP Baptista, W Xiao, EM Hall… - Genome …, 2021 - genome.cshlp.org
Mitochondrial genome content and structure vary widely across the eukaryotic tree of life,
with protists displaying extreme examples. Apicomplexan and dinoflagellate protists have …

Rapid mitochondrial genome sequencing based on Oxford Nanopore Sequencing and a proxy for vertebrate species identification

ND Franco‐Sierra, JF Díaz‐Nieto - Ecology and Evolution, 2020 - Wiley Online Library
Molecular information is crucial for species identification when facing challenging
morphology‐based specimen identifications. The use of DNA barcodes partially solves this …

Are we there yet? Benchmarking low-coverage nanopore long-read sequencing for the assembling of mitochondrial genomes using the vulnerable silky shark …

JA Baeza, FJ García-De León - BMC genomics, 2022 - Springer
Background Whole mitochondrial genomes are quickly becoming markers of choice for the
exploration of within-species genealogical and among-species phylogenetic relationships …

Sequencing and reconstructing helminth mitochondrial genomes directly from genomic next-generation sequencing data

N Palevich, PH Maclean - Parasite Genomics: Methods and Protocols, 2021 - Springer
We present a detailed method for extraction of high-molecular weight genomic DNA suitable
for numerous DNA sequencing applications, and a straightforward in silico approach for …

Highly-multiplexed and efficient long-amplicon PacBio and Nanopore sequencing of hundreds of full mitochondrial genomes

BR Karin, S Arellano, L Wang, K Walzer, A Pomerantz… - BMC genomics, 2023 - Springer
Background Mitochondrial genome sequences have become critical to the study of
biodiversity. Genome skimming and other short-read based methods are the most common …