A universal approach to optimize the folding and stability of prefusion-closed HIV-1 envelope trimers
The heavily glycosylated native-like envelope (Env) trimer of HIV-1 is expected to have low
immunogenicity, whereas misfolded forms are often highly immunogenic. High-quality …
immunogenicity, whereas misfolded forms are often highly immunogenic. High-quality …
Automated design by structure-based stabilization and consensus repair to achieve prefusion-closed envelope trimers in a wide variety of HIV strains
Soluble envelope (Env) trimers, stabilized in a prefusion-closed conformation, can elicit
neutralizing responses against HIV-1 strains closely related to the immunizing trimer …
neutralizing responses against HIV-1 strains closely related to the immunizing trimer …
Soluble prefusion closed DS-SOSIP. 664-Env trimers of diverse HIV-1 strains
MG Joyce, IS Georgiev, Y Yang, A Druz, H Geng… - Cell reports, 2017 - cell.com
The elicitation of autologous neutralizing responses by immunization with HIV-1 envelope
(Env) trimers conformationally stabilized in a prefusion closed state has generated …
(Env) trimers conformationally stabilized in a prefusion closed state has generated …
Uncleaved prefusion-optimized gp140 trimers derived from analysis of HIV-1 envelope metastability
The trimeric HIV-1 envelope glycoprotein (Env) is critical for host immune recognition and
neutralization. Despite advances in trimer design, the roots of Env trimer metastability …
neutralization. Despite advances in trimer design, the roots of Env trimer metastability …
Rational design of DNA-expressed stabilized native-like HIV-1 envelope trimers
Summary The HIV-1-envelope glycoprotein (Env) is the main target of antigen design for
antibody-based prophylactic vaccines. The generation of broadly neutralizing antibodies …
antibody-based prophylactic vaccines. The generation of broadly neutralizing antibodies …
Structure-based design of a soluble prefusion-closed HIV-1 Env trimer with reduced CD4 affinity and improved immunogenicity
ABSTRACT The HIV-1 envelope (Env) trimer is a target for vaccine design as well as a
conformational machine that facilitates virus entry by transitioning between prefusion-closed …
conformational machine that facilitates virus entry by transitioning between prefusion-closed …
Functional and highly cross-linkable HIV-1 envelope glycoproteins enriched in a pretriggered conformation
Binding to the receptor, CD4, drives the pretriggered,“closed”(state-1) conformation of the
human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) trimer into more …
human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) trimer into more …
Structure-based design of native-like HIV-1 envelope trimers to silence non-neutralizing epitopes and eliminate CD4 binding
Elicitation of broadly neutralizing antibodies (bnAbs) is a primary HIV vaccine goal. Native-
like trimers mimicking virion-associated spikes present nearly all bnAb epitopes and are …
like trimers mimicking virion-associated spikes present nearly all bnAb epitopes and are …
Improving the immunogenicity of native-like HIV-1 envelope trimers by hyperstabilization
The production of native-like recombinant versions of the HIV-1 envelope glycoprotein (Env)
trimer requires overcoming the natural flexibility and instability of the complex. The …
trimer requires overcoming the natural flexibility and instability of the complex. The …
Structure-guided redesign improves NFL HIV Env trimer integrity and identifies an inter-protomer disulfide permitting post-expression cleavage
L Yang, SK Sharma, C Cottrell, J Guenaga… - Frontiers in …, 2018 - frontiersin.org
Soluble HIV-1 envelope glycoprotein (Env) trimers are under active investigation as vaccine
candidates in relevant pre-clinical models. Like SOSIPs, the cleavage-independent native …
candidates in relevant pre-clinical models. Like SOSIPs, the cleavage-independent native …