The advent of affordable high-throughput DNA sequencing has opened up a golden age of
studies in the human microbiome. In order to understand the role of the human microbiota …

There is a growing appreciation for the role of microbial communities as critical modulators
of human health and disease. High throughput sequencing technologies have allowed for …

Background New developments in next-generation sequencing technologies and massive
data received from this approach open wide prospects for personalised medicine and …

Microbiome studies are becoming larger in size to detect the potentially small effect that
environmental factors have on our gut microbiomes, or that the microbiome has on our …

The human microbiome has emerged as a major player in regulating human health and
disease. Translational studies of the microbiome have the potential to indicate clinical …

The gut microbiome is widely analyzed using high-throughput sequencing, such as 16S
rRNA gene amplicon sequencing and shotgun metagenomic sequencing (SMS). DNA …

Microbial community analysis can be biased by multiple technical factors, such as storage
conditions, DNA extraction, or amplification conditions. In a high-throughput laboratory that …

Observations from human microbiome studies are often conflicting or inconclusive. Many
factors likely contribute to these issues including small cohort sizes, sample collection, and …

Rapid advancements in sequencing technologies along with falling costs present
widespread opportunities for microbiome studies across a vast and diverse array of …

Introduction: Since the discovery of Polymerase Chain Reaction and pioneering work on
16S ribosomal RNA genes in the 1980's, the use of molecular techniques to study microbial …