The Escherichia coli protein DnaA and the plasmid RK2-encoded TrfA protein are required
for initiation of replication of the broad host range plasmid RK2. The TrfA protein has been …

Specific binding of the plasmid-encoded protein, TrfA, and the Escherichia coli DnaA protein
to the origin region (oriV) is required for the initiation of replication of the broad host range …

The 393-base pair minimal origin, oriV, of plasmid RK2 contains three iterated motifs
essential for initiation of replication: consensus sequences for binding the bacterial DnaA …

The TrfA proteins, encoded by the broad host range plasmid RK2, are required for
replication of this plasmid in a variety of Gram-negative bacteria. Two TrfA proteins, 33 and …

The TrfA protein encoded by the broad host range bacterial plasmid RK2 specifically binds
to eight direct repeats (iterons) present at the plasmid replication origin to initiate DNA …

Replication of plasmid RK2 in a variety of Gram-negative bacteria requires its origin of
replication and the plasmid-encoded TrfA proteins (TrfA-33 and TrfA-44). The initiation of …

The broad-host-range plasmid RK2 is capable of replication and stable maintenance within
a wide range of gram-negative bacterial hosts. It encodes the essential replication initiation …

The DnaA replication initiation protein has been shown to be essential for DNA strand
opening at the AT-rich region of the replication origin of the Escherichia coli chromosome as …

The broad host range plasmid RK2 replicates and regulates its copy number in a wide range
of Gram-negative bacteria. The plasmid-encoded trans-acting replication protein TrfA and …

Initiation and control of replication of the broad-host-range plasmid RK2 requires two
plasmid-encoded elements, the replication origin (oriV) and the initiation protein TrfA …