Publisher Summary The first transgenic mouse was created in the early 1990s via
incubation of spermatozoa with exogenous DNA followed by in vitro fertilization (IVF). The …

Sperm‐mediated DNA transfer to offspring has the potential to markedly simplify the
generation of transgenic animals, but the efficiency in mice has been controversial. To …

The idea of using a sperm cell to introduce exogenous DNA into an oocyte at the time of
fertilization is of interest for simple production of transgenic mice. Since 1989, contradictory …

Improvement of techniques in molecular biology and mammalian embryo manipulation have
recently made possible the production of transgenic animals. These technologies offer …

The production of transgenic animals by sperm cell-mediated gene transfer continues to be
a fascinating and intriguing possibility due to the simplicity and potential use of the …

In the field of animal transgenesis, many attempts have been made recently to simplify
facilitate, and reduce the cost and labor required to do such tasks. Although several …

A meeting entitled 'Sperm-mediated gene transfer: advances in sperm cell research and
applications' was held on May 23–26, 1999 at the University of Siena, Certosa di …

The possibility of sperm as a vehicle to deliver foreign DNA to oocytes was tested in
hamsters. Epididymal spermatozoa, incubated with linearized plasmid DNA encoding ovine …

The transgenic technologies represent potent biotechnological tools that allow the
generation of genetically modified animals useful for basic research and for biomedical …

Sperm‐mediated gene transfer in vertebrates has undergone various developments over
the last few years, in different laboratories. In the present study, we microinjected a circular …