Design and application of single-cell RNA sequencing to study kidney immune cells in lupus nephritis

DA Rao, A Arazi, D Wofsy, B Diamond - Nature Reviews Nephrology, 2020 - nature.com
… We also discuss current findings from single-cell RNA sequencing (scRNA-seq) analyses
of lupus nephritis kidneys and urine, including the immune cell populations identified and how …

Advantages of single-nucleus over single-cell RNA sequencing of adult kidney: rare cell types and novel cell states revealed in fibrosis

H Wu, Y Kirita, EL Donnelly… - Journal of the American …, 2019 - journals.lww.com
RNA sequencing (snRNA-seq) datasets (n=3). This revealed 20-fold more podocytes
from snRNA-seq (2.4%) compared with single-cell RNA sequencing (scRNA-seq; 0.12%; P=0.02). …

Single-cell RNA sequencing for the study of development, physiology and disease

SS Potter - Nature Reviews Nephrology, 2018 - nature.com
… In the developing mouse or human kidney, for example, cap mesenchyme progenitor cells
can be identified by their expression of Cited1, Six2, Eya1 and Osr1; stromal progenitor cells …

Defining housekeeping genes suitable for RNA-seq analysis of the human allograft kidney biopsy tissue

Z Wang, Z Lyu, L Pan, G Zeng, P Randhawa - BMC medical genomics, 2019 - Springer
… To generate a HKG set specific for kidney transplantation, we performed RNA-sequencing
from renal allograft biopsies collected in a variety of clinical settings. Various normalization …

Single-cell RNA sequencing identifies candidate renal resident macrophage gene expression signatures across species

KA Zimmerman, MR Bentley, JM Lever… - Journal of the …, 2019 - journals.lww.com
… cluster of cells in rat, pig, and human kidney tissue that had enrichment of several of the …
C1q, whose expression was enriched at the RNA level in the candidate resident macrophage …

Single-cell RNA sequencing reveals the mesangial identity and species diversity of glomerular cell transcriptomes

B He, P Chen, S Zambrano, D Dabaghie, Y Hu… - Nature …, 2021 - nature.com
… To see whether this occurred also in human MCs, we isolated glomeruli from human
kidneys and performed in vitro phagocytosis assay in PDGFRB + cells. When primary …

Single cell RNA sequencing identifies a unique inflammatory macrophage subset as a druggable target for alleviating acute kidney injury

W Yao, Y Chen, Z Li, J Ji, A You, S Jin, Y Ma… - Advanced …, 2022 - Wiley Online Library
… [ 16 ] Single-cell RNA sequencing (scRNA-seq) opens the possibility to … human kidney and
in kidneys across species and myeloid cells during regression of fibrosis in the mouse kidney. …

Differentially expressed gene transcripts using RNA sequencing from the blood of immunosuppressed kidney allograft recipients

C Dorr, B Wu, W Guan, A Muthusamy, K Sanghavi… - PloS one, 2015 - journals.plos.org
… We performed RNA sequencing (RNAseq) on peripheral blood mononuclear cells (PBMCs) …
) after kidney transplantation and after the start of immunosuppressive drugs. RNAseq is …

Molecular characterization of the human kidney interstitium in health and disease

…, MT Eadon, Kidney Precision Medicine Project - Science …, 2021 - science.org
RNA sequencing (snDrop RNAseq) (12). In an overlapping set of nephrectomies and
renal biopsy specimens, spatial context was provided for these single nuclear signatures by …

[HTML][HTML] Single nucleotide variant counts computed from RNA sequencing and cellular traffic into human kidney allografts

G Thareja, H Yang, S Hayat, FB Mueller, JR Lee… - American Journal of …, 2018 - Elsevier
RNA sequence data of kidney allograft biopsies provide an objective measure of cellular
infiltration of the allograft. We performed mRNA sequencing of 40 kidney … the sequencing reads …