Direct comparison of SARS-CoV-2 analytical limits of detection across seven molecular assays

B Fung, A Gopez, V Servellita, S Arevalo… - Journal of clinical …, 2020 - Am Soc Microbiol
… Using pooled patient material quantified with ddPCR, it is reassuring that measured limits of
… these patients would likely have developed detectable antibody responses by this time, and …

[HTML][HTML] Seroepidemiologic study designs for determining SARS-COV-2 transmission and immunity

H Clapham, J Hay, I Routledge… - Emerging infectious …, 2020 - ncbi.nlm.nih.gov
… and antibody kinetics. The dashed horizontal line represents the limit of detection of the
assay. Early on, viral loads are more sensitive for diagnosing recent infection, whereas antibody

[HTML][HTML] Detection, prevalence, and duration of humoral responses to SARS-CoV-2 under conditions of limited population exposure

TJ Ripperger, JL Uhrlaub, M Watanabe, R Wong… - medRxiv, 2020 - ncbi.nlm.nih.gov
… to estimate the durability of antibody production and immunity after SARS-CoV-2 infections.
… Although most N titers did not fall fully below our detection limits, we also observed such a …

Rapid and quantitative detection of SARS-CoV-2 specific IgG for convalescent serum evaluation

X Tan, M Krel, E Dolgov, S Park, X Li, W Wu… - Biosensors and …, 2020 - Elsevier
… sensitive detection of SARS-CoV-2 antigens (S1 and N proteins) with a lower limit of detection
(LLOD) of ~… To compare the antibody's affinity towards the S1 protein of SARS-CoV-2 and …

Robust validation and performance comparison of immunogenicity assays assessing IgG and neutralizing antibodies to SARS-CoV-2

ME Bonhomme, CJ Bonhomme, L Strelow… - PLoS …, 2022 - journals.plos.org
… activity and amount of antibodies against the spike and RBD … immune response and antibody
titers following SARS-CoV-2 … limits of quantification (LOQ) and limits of detection (LOD). …

Usefulness and Limitations of Anti-S IgG Assay in Detecting Previous SARS-CoV-2 Breakthrough Infection in Fully Vaccinated Healthcare Workers

G Spiteri, MGL Monaco, G Caliskan, A Carta… - Diagnostics, 2022 - mdpi.com
antibody titer assessment and previous SARS-CoV-2 infection. Accuracy (AUC) sensitivity
increased from 0.78 (95% CI 0.70–0.85) for BI in the previous six months to 0.83 (95% CI 0.67…

Anti-SARS-CoV-2 antibodies testing in recipients of COVID-19 vaccination: why, when, and how?

G Lippi, BM Henry, M Plebani - Diagnostics, 2021 - mdpi.com
limits, different cut-offs, and dissimilar ranges of antibody concentrations. This shall hence
be seen as a tangible hurdle in setting a universal approach for "personalized vaccination.” …

[HTML][HTML] Antibody response to SARS-CoV-2 for more than one year− kinetics and persistence of detection are predominantly determined by avidity progression and …

H Scheiblauer, CM Nübling, T Wolf… - Journal of clinical …, 2022 - Elsevier
… This correlated with persistent antibody detection depending on antibody-binding efficiency
… of anti-SARS-CoV-2 assays was determined by test design, target antigen, antibody avidity, …

A highly specific and sensitive serological assay detects SARS-CoV-2 antibody levels in COVID-19 patients that correlate with neutralization

D Peterhoff, V Glück, M Vogel, P Schuster, A Schütz… - Infection, 2021 - Springer
… Of note, mild infections resulted in short-lived and very low antibody titers near the detection
limit. This loss of humoral immunity has been linked to the occurrence of (re-)infections with …

Evaluation of three quantitative anti-SARS-CoV-2 antibody immunoassays

S Chapuy-Regaud, M Miédougé… - Microbiology …, 2021 - Am Soc Microbiol
… acute respiratory syndrome coronavirus 2 (SARS-CoV-2) … antibodies directed to the
SARS-CoV-2 Spike (S) antigen, either total (Wantaï SARS-CoV-2 Ab ELISA) or IgG (SARS-CoV-2 …