SARS-CoV-2 pathogenesis

MM Lamers, BL Haagmans - Nature reviews microbiology, 2022 - nature.com
expressing high levels of HLA-DR, whereas patients with severe COVID-19 had monocytes
expressing … strongly decreased mRNA and protein expression of the receptor tyrosine kinase …

Recombinant protein expression and purification of N, S1, and RBD of SARS-CoV-2 from mammalian cells and their potential applications

J Garcia-Cordero, J Mendoza-Ramirez… - Diagnostics, 2021 - mdpi.com
… In this study, we designed and evaluated an expression system for the S1 and N proteins
and estimated the level of purification of two recombinant proteins. Our results should be useful …

[HTML][HTML] Silencing SARS-CoV Spike protein expression in cultured cells by RNA interference

Y Zhang, T Li, L Fu, C Yu, Y Li, X Xu, Y Wang, H Ning… - FEBS letters, 2004 - Elsevier
… vitro expression system of the Spike protein from SARS-CoV, … protein expression in cells
transfected with the expression … to be susceptible to SARS-CoV infection and replication. We …

The SARS-CoV nucleocapsid protein: a protein with multifarious activities

M Surjit, SK Lal - Infection, genetics and evolution, 2008 - Elsevier
… processes perturbed by heterologous expression of the N-protein are discussed below. … of
different SARS-CoV proteins, which revealed that ORF3b, ORF6 as well as the N-protein have …

Expression and characterization of SARS-CoV-2 spike proteins

JM Schaub, CW Chou, HC Kuo, K Javanmardi… - Nature protocols, 2021 - nature.com
… Here, we describe the expression, purification and biophysical characterization of HexaPro,
a second-generation prefusion-stabilized SARS-CoV-2 spike glycoprotein from mammalian …

The SARS-CoV S glycoprotein: expression and functional characterization

X Xiao, S Chakraborti, AS Dimitrov… - Biochemical and …, 2003 - Elsevier
… To characterize the properties and function of the SARS-CoV S protein we cloned in expression
vectors the DNAs encoding the full-length Tor2 isolate [6] S protein (1255 residues), the …

Gene expression and in situ protein profiling of candidate SARS-CoV-2 receptors in human airway epithelial cells and lung tissue

JA Aguiar, BJM Tremblay, MJ Mansfield… - European …, 2020 - Eur Respiratory Soc
protein expression in the airway epithelium and alveoli of human lung. Our protein expression
… epithelial cell samples and low ACE2 gene expression in both microarray and single cell …

Expression and purification of SARS coronavirus proteins using SUMO-fusions

X Zuo, MR Mattern, R Tan, S Li, J Hall… - Protein expression and …, 2005 - Elsevier
… protease (3CL), and membrane (M), have become the focus of efforts to produce antiviral
agents and vaccines against SARS. The SARS-CoV proteins investigated in this study are …

SARScoronavirus modulation of myocardial ACE2 expression and inflammation in patients with SARS

GY Oudit, Z Kassiri, C Jiang, PP Liu… - European journal of …, 2009 - Wiley Online Library
… of SAR-CoV in the hearts could be associated with decreased ACE2 protein expression. …
for myocardial ACE2 protein expression showed that the presence SARS-CoV in the heart …

Construction of SARS-CoV-2 virus-like particles by mammalian expression system

R Xu, M Shi, J Li, P Song, N Li - Frontiers in bioengineering and …, 2020 - frontiersin.org
… of four SARS-CoV-2 structural proteins, we first transfected HEK-293T and Vero E6 cells
with vectors expressing S, M, E, or N, respectively. As shown in Figures 2A,B, M protein was …