Duration of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infectivity: when is it safe to discontinue isolation?

C Rhee, S Kanjilal, M Baker… - Clinical infectious …, 2021 - academic.oup.com
… associated with replication-competent virus thus far is 20 … of SARS-CoV-2, and how this
has informed evolving public health recommendations on when it is safe to discontinue isolation

To interpret the SARS-CoV-2 test, consider the cycle threshold value

MR Tom, MJ Mina - Clinical Infectious Diseases, 2020 - academic.oup.com
… In summary, prolonged positive SARSCoV-2 RT-qPCR results raise questions about the
sufficiency and sustainability of current isolation guidelines. We suggest that the Ct value from …

Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model

TF Rogers, F Zhao, D Huang, N Beutler, A Burns, W He… - Science, 2020 - science.org
… Here, we present the isolation of highly potent nAbs to SARS-CoV-2 and demonstrate their
… against SARS-CoV-2 and discover nAbs, we adapted our pipeline to rapidly isolate and …

SARS-CoV-2 isolation from ocular secretions of a patient with COVID-19 in Italy with prolonged viral RNA detection

F Colavita, D Lapa, F Carletti, E Lalle… - Annals of internal …, 2020 - acpjournals.org
CoV-2 Infection Detected in Italy* Discussion: We found that ocular fluids from SARS-CoV-2–infected
patients may contain infectious virus… not only a site of virus entry but also a source …

[HTML][HTML] SARS-CoV-2 is transmitted via contact and via the air between ferrets

M Richard, A Kok, D de Meulder, TM Bestebroer… - Nature …, 2020 - nature.com
… Individually housed donor ferrets were inoculated intranasally with a strain of SARS-CoV-2
isolated from a German traveller returning from China. Six hours post-inoculation (hpi), a …

Cell-based culture of SARS-CoV-2 informs infectivity and safe de-isolation assessments during COVID-19

K Basile, K McPhie, I Carter, S Alderson, H Rahman… - medRxiv, 2020 - medrxiv.org
… We demonstrate that virus with replicative capacity can be isolated and detected in the …
samples where SARS-CoV-2 was detected. This effect wasn’t due to a known SARS-CoV-2 …

[HTML][HTML] SARS-CoV and SARS-CoV-2 are transmitted through the air between ferrets over more than one meter distance

JS Kutter, D de Meulder, TM Bestebroer… - Nature …, 2021 - nature.com
… The SNP analysis of the virus isolate used to inoculate ferrets (passage 3 of the virus
stock) revealed that only the substitution S686G was present in >5% of the reads (Table S1B). …

Transmission of SARS-CoV-2: a review of viral, host, and environmental factors

EA Meyerowitz, A Richterman, RT Gandhi… - Annals of internal …, 2021 - acpjournals.org
… live virus has been isolated from saliva and stool and viral … evidence about the transmission
of SARS-CoV-2, we attempt to … virus in experimental and real-world settings? Second, what …

[HTML][HTML] Systematic comparison of two animal-to-human transmitted human coronaviruses: SARS-CoV-2 and SARS-CoV

J Xu, S Zhao, T Teng, AE Abdalla, W Zhu, L Xie… - Viruses, 2020 - mdpi.com
… The source of unexplained pneumonia was first discovered in Wuhan in Dec, 2019, and
SARS-CoV-2, a new coronavirus, was isolated from the respiratory epithelium of patients. It …

[HTML][HTML] Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein

SJ Zost, P Gilchuk, RE Chen, JB Case, JX Reidy… - Nature medicine, 2020 - nature.com
… Using the parallel workflows, we isolated 389 recombinant SARS-CoV-2-reactive human
mAbs that expressed sufficiently well as recombinant IgG to characterize the activity of the mAb. …