[HTML][HTML] A simplified SARS-CoV-2 detection protocol for research laboratories

S Paz, C Mauer, A Ritchie, JD Robishaw, M Caputi - PLoS One, 2020 - journals.plos.org
… To evaluate the simplified TRIzol method in isolating viral RNA from NP, OP, NV and saliva
… of inactivated SARS-CoV-2 virus (isolate USA-WA1/2020) containing 10,000 viral genome …

An analysis of SARS-CoV-2 viral load by patient age

TC Jones, B Mühlemann, T Veith, G Biele… - MedRxiv, 2020 - medrxiv.org
… a log 10 viral load below 5.4 (∼250,000 copies per mL) have a probability of yielding a
virus isolate below 5%. We also found that virus could not be isolated from respiratory samples …

Cycle threshold values in RT‐PCR to determine dynamics of SARSCoV‐2 viral load: An approach to reduce the isolation period for COVID‐19 patients

C Aranha, V Patel, V Bhor… - Journal of medical …, 2021 - Wiley Online Library
… live virus is relatively short-lived. In this study, we compared the duration of SARS-CoV-2 viral
… and evaluated the dynamics of viral load in cases that required 15–30 days to clear SARS-…

Detection and isolation of airborne SARSCoV‐2 in a hospital setting

N Rufino de Sousa, L Steponaviciute, L Margerie… - Indoor …, 2022 - Wiley Online Library
… for SARS-CoV-2 RNA, it remained to be determined whether active virus particles could
be recovered from the same air-sample material. Thus, we attempted to isolate SARS-CoV-2 …

[HTML][HTML] Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards

B La Scola, M Le Bideau, J Andreani, VT Hoang… - European Journal of …, 2020 - Springer
… infectious viral particles. It was observed that SARS-CoV-2 was detected up to 20 days after
onset of symptoms by PCR in infected patients but that the virus could not be isolated after …

[HTML][HTML] SARS-CoV-2 infections and viral isolations among serially tested cats and dogs in households with infected owners in Texas, USA

SA Hamer, A Pauvolid-Corrêa, IB Zecca, E Davila… - Viruses, 2021 - mdpi.com
… of coronavirus disease 2019 (COVID-19), caused by the novel severe acute respiratory
syndrome virus 2 (SARS-CoV-2), … SARS-CoV-2 and its closest relatives suggest the reservoir of …

Persistent viral shedding of SARSCoV‐2 in faeces–a rapid review

S Gupta, J Parker, S Smits, J Underwood… - Colorectal …, 2020 - Wiley Online Library
… Similar patterns of virus isolation from stool and faeco-oral transmission were observed for
other coronaviruses including SARS-CoV-1 [34]. Bio-aerosol generation of viral particles as a …

Clinical and laboratory diagnosis of SARS-CoV-2, the virus causing COVID-19

SJR da Silva, CTA Silva, KM Guarines… - ACS infectious …, 2020 - ACS Publications
coronavirus) (12) and 50% to MERS-CoV. The most closely related virus to SARS-CoV-2
found so far is a CoV isolated from bats, named RaGT13 CoV, whose nucleotide identity is 96%…

[HTML][HTML] Challenges in laboratory diagnosis of the novel coronavirus SARS-CoV-2

N Younes, DW Al-Sadeq, H Al-Jighefee, S Younes… - Viruses, 2020 - mdpi.com
SARS-CoV-2 is dependent upon the understanding of the proteomic and genomic composition
of the virus and the viral … , 104 strains of SARS-CoV-2 virus were isolated and sequenced …

[HTML][HTML] Laboratory diagnosis of SARS-CoV-2-A review of current methods

JP Mathuria, R Yadav - Journal of infection and public health, 2020 - Elsevier
… Researcher from wuhan, china have done virus isolation on human airway … isolated virus
was named 2019-nCoV [19]. Using the human airway epithelial cell cultures for virus isolation