… one-step reverse transcription loop-mediated isothermal amplification assay for rapid identification of RNA from the severe fever with thrombocytopenia syndrome …

H Xu, L Zhang, G Shen, C Feng, X Wang, J Yan… - Journal of Virological …, 2013 - Elsevier
… RT-LAMP assay established in this study perceives all these characteristics. This amplification
… The RT-LAMP reaction allows for target sequence amplification at isothermal condition …

Evaluation of reverse transcription loop‐mediated isothermal amplification assay for the detection of severe fever with thrombocytopenia syndrome in clinical …

W Tian, Y Zhang, S Geng, J Wang, W Ji… - Journal of Medical …, 2023 - Wiley Online Library
… Detection of severe fever with thrombocytopenia syndrome (SFTS) by using reverse
transcription loop-mediated isothermal amplification (RT-LAMP). The RT-LAMP products of primer …

Developing a multiplex loop-mediated isothermal amplification assay (LAMP) to determine severe fever with thrombocytopenia syndrome (SFTS) and scrub typhus

WS Jang, DH Lim, YL Choe, J Nam, KC Moon, C Kim… - Plos one, 2022 - journals.plos.org
Severe fever with thrombocytopenia syndrome (SFTS) and … The causative pathogen of
SFTS is severe fever … The clinical features of SFTS are fever, headache, myalgia, …

Multiplex nucleic acid amplification test for diagnosis of dengue fever, malaria, and leptospirosis

JJ Waggoner, J Abeynayake, I Balassiano… - Journal of clinical …, 2014 - Am Soc Microbiol
… The specificity of the UFI assay was evaluated by testing … No amplification was observed
when the UFI assay was … otherwise showed no amplification in the UFI assay. Fifty HCV-…

Development of a real-time loop-mediated isothermal amplification method for the detection of severe fever with thrombocytopenia syndrome virus

JW Lee, YJ Won, LH Kang, SG Lee, SW Park… - Journal of …, 2020 - Springer
… Here, we designed novel primers for this assay and found that the technique had very high
… isothermal amplification assay for rapid detection of a new SFTS bunyavirus. Arch. Virol. …

Detection of severe fever with thrombocytopenia syndrome virus by reverse transcription–cross-priming amplification coupled with vertical flow visualization

L Cui, Y Ge, X Qi, G Xu, H Li, K Zhao… - Journal of clinical …, 2012 - Am Soc Microbiol
assay detected only one of three 10-copy/reaction tubes. The results indicate that the RT-CPA-VF
assay … -CPA-VF assay was evaluated by RT-CPA-VF amplification of viral RNA extracts …

[HTML][HTML] Severe fever with thrombocytopenia syndrome: a newly discovered emerging infectious disease

DX Li - Clinical Microbiology and Infection, 2015 - Elsevier
… limit of the assay is 100 copies/mL or 1 TCID 50 /mL [53]. A loop-mediated isothermal
amplification assay targeting the S segment of SFTSV has been developed; with this assay, the …

Development and preliminary evaluation of a multiplexed amplification and next generation sequencing method for viral hemorrhagic fever diagnostics

A Brinkmann, K Ergünay, A Radonić… - PLoS neglected …, 2017 - journals.plos.org
… to cause hemorrhagic fever in humans. The method relies on selected amplification of the
… have demonstrated excellent suitability of the assay in various sequencing platforms, which …

… evaluation of a rapid detection assay for severe fever with thrombocytopenia syndrome virus based on reverse-transcription recombinase polymerase amplification

J Zhou, Q Wang, L Zhu, S Li, W Li, Y Fu… - Molecular and cellular …, 2020 - Elsevier
… polymerase amplification (RT-RPA) assay was … suspected severe fever with thrombocytopenia
syndrome (SFTS) patients revealed that the sensitivity and specificity of the RT-RPA assay

Viral hemorrhagic fever diagnostics

LD Racsa, CS Kraft, GG Olinger… - Clinical Infectious …, 2016 - academic.oup.com
amplification (… assay that has been developed for RVFV produces results within 2 hours,
including RNA extraction [31]. LAMP assays have been developed for detection of severe fever