L Premkumar, B Segovia-Chumbez, R Jadi… - Science …, 2020 - science.org
… -based diagnostic assays, we focused on the RBD domain of the spike protein because this … target of human antibodies (19). A major concern with using a protein domain instead of a full…
Tools to monitor SARS-CoV-2 transmission and immune responses are needed. We present a neutralization ELISA to determine the levels of Ab-mediated virus neutralization and a …
AS Iyer, FK Jones, A Nodoushani, M Kelly… - Science …, 2020 - science.org
… A detailed characterization of antibody responses is needed to determine whether antibody-… the kinetics and antibody isotype profile to the receptor binding domain (RBD) of the spike (S…
JR Byrnes, XX Zhou, I Lui, SK Elledge, JE Glasgow… - MSphere, 2020 - Am Soc Microbiol
… SARS-CoV-2 Spike protein and determine the proportion of anti-Spikeantibodies that block … ), we present natively folded viral Spike protein receptor-binding domain (RBD)-containing …
Z Cao, L Liu, L Du, C Zhang, S Jiang, T Li, Y He - Virology journal, 2010 - Springer
… showed that the spike RBD could induce potent and persistent antibody responses in the … in the immunoaffinity column, the anti-RBD antibodies purified from the positive human serum …
Y Wu, C Li, S Xia, X Tian, Y Kong, Z Wang, C Gu… - Cell host & …, 2020 - cell.com
… receptor engagement, is a potential therapeutic target. We describe the development of a phage-displayed single-domainantibody … cryptic epitope located in the spike trimeric interface. …
J Sui, M Deming, B Rockx, RC Liddington… - Journal of …, 2014 - Am Soc Microbiol
The receptor binding domain (RBD) of the spike (S) glycoprotein of severe acute respiratory syndrome coronavirus (SARS-CoV) is a major target of protective immunity in vivo. Although …
L Du, G Zhao, Y Yang, H Qiu, L Wang, Z Kou… - Journal of …, 2014 - Am Soc Microbiol
… a neutralizing monoclonal antibody (MAb)… receptor-binding domain (RBD) of the MERS-CoV spike protein and thereby competitively blocks the binding of the RBD to its cellular receptor…
… 2 spike (S) and nucleoprotein and nAb titers correlate with clinical scores. The receptor-binding domain (… Our results explain the immunodominance of the receptor-binding motif and will …