Readfish enables targeted nanopore sequencing of gigabase-sized genomes

A Payne, N Holmes, T Clarke, R Munro… - Nature …, 2021 - nature.com
… Finally, we enrich targeted panels comprising 25,600 exons from 10,000 human genes and
… in <15 h sequencing. These methods can be used to efficiently screen any target panel of …

Targeted nanopore sequencing with Cas9-guided adapter ligation

T Gilpatrick, I Lee, JE Graham, E Raimondeau… - Nature …, 2020 - nature.com
… describe nanopore Cas9-targeted sequencing (nCATS), an enrichment strategy that uses
targeted cleavage of chromosomal DNA with Cas9 to ligate adapters for nanopore sequencing

Targeted nanopore sequencing by real-time mapping of raw electrical signal with UNCALLED

S Kovaka, Y Fan, B Ni, W Timp, MC Schatz - Nature biotechnology, 2021 - nature.com
targeted sequencing methods eliminate many of the benefits of nanopore sequencing, such
… The ReadUntil method allows nanopore devices to selectively eject reads from pores in real …

Nanopore targeted sequencing for the accurate and comprehensive detection of SARS‐CoV‐2 and other respiratory viruses

M Wang, A Fu, B Hu, Y Tong, R Liu, Z Liu, J Gu… - Small, 2020 - Wiley Online Library
… in-house, followed by sequencing of the amplified fragments on a nanopore platform. To
enhance sensitivity, we focused on virulence-related genes as targets without limitation of the …

Clinical performance of nanopore targeted sequencing for diagnosing infectious diseases

Y Fu, Q Chen, M Xiong, J Zhao, S Shen… - Microbiology …, 2022 - Am Soc Microbiol
targeted antimicrobial therapy and reduce overuse of broad-spectrum antibiotics. Nanopore
targeted sequencing … and PCR followed by Sanger sequencing. This retrospective study was …

Comprehensive genetic diagnosis of tandem repeat expansion disorders with programmable targeted nanopore sequencing

I Stevanovski, SR Chintalaphani, H Gamaarachchi… - Science …, 2022 - science.org
targeted long-read sequencing with Oxford Nanopore’s … Targeted long-read sequencing
solves large and complex … and short-read sequencing and identifies noncanonical STR …

Nanopore targeted sequencing for rapid gene mutations detection in acute myeloid leukemia

C Cumbo, CF Minervini, P Orsini, L Anelli, A Zagaria… - Genes, 2019 - mdpi.com
… recently it was validated a 19-gene AML-targeted NGS panel that could be a valid approach
to … Our work shows that the long-reads nanopore sequencing approach for gene mutation …

Pan-genomic matching statistics for targeted nanopore sequencing

O Ahmed, M Rossi, S Kovaka, MC Schatz, T Gagie… - Iscience, 2021 - cell.com
Nanopore sequencing is an increasingly powerful tool for genomics. Recently, computational
advances have allowed nanopores to sequence in a targeted fashion; as the sequencer

Selective nanopore sequencing of human BRCA1 by Cas9-assisted targeting of chromosome segments (CATCH)

T Gabrieli, H Sharim, D Fridman, N Arbib… - Nucleic acids …, 2018 - academic.oup.com
… for targeted nanopore sequencing and showed an enrichment factor of 21.7 for sequencing
of a 200 kb target … Here, we harness CATCH for nanopore and NGS sequencing of human …

Potential utility of targeted Nanopore sequencing for improving etiologic diagnosis of bacterial and fungal respiratory infection

WS Chan, CH Au, SM Leung, DN Ho, EYL Wong… - Diagnostic …, 2020 - Springer
… In this study, we explored the use of Nanopore sequencing as a supplementary … targeted
Nanopore method is an intermediate between the two, in terms of speed (FilmArray > Nanopore