… images. To address both challenges, we developed Virtual Alignment of pathoLogy Image
Series (VALIS), software which enables generation of highly multiplexed images by aligning …

… image registration methods for whole slide images (WSI), most have specific use cases. Here,
we present the Virtual Alignment of pathoLogy Image Series … ), and saves aligned slides in …

… in breast pathology [1], gastrointestinal tract pathology [2], … in the acquired virtual slices and
the large size of these images, … steps roughly align the images in consecutive image slides. …

… unnecessary to align an entire image when the bulk of the image is … large-scale image
registration issues in histopathology and … between two images is optimized after a series of affine …

… In response to this gap and in alignment with our … and robustness of virtual staining
techniques in histopathology, we … After cycling through all S stains in a random sequence, we …

… registration with global alignment using a computationally … On datasets of simulated video
scans of pathology slides, we … On extracting a sequence of images from video, we compute …

… By using the image analysis software we have described, rou… are aligned correctly with
respect to each other (Köhler 1893). … in a digital image–a two-dimensional sequence of pixel …

… image-to-image translation methods require aligned or … different aspects of virtual
histopathology staining including … of the generated virtual histopathology images remains …

… We efficiently align consecutive slides at increasingly fine resolution … Thorough visual
inspection of virtual cuts through the … a given a series of consecutive histological images I 1 , …, I …

… was available in each level of the whole-slide image series; and (4) for nonglobal stained …
the current method to align consecutive, whole-slide gigapixel histopathology images, thereby …