LoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions

D Shaw, S Miravet‐Verde… - Microbial …, 2021 - Wiley Online Library
The removal of unwanted genetic material is a key aspect in many synthetic biology efforts
and often requires preliminary knowledge of which genomic regions are dispensable …

LoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions.

D Shaw, S Miravet‐Verde… - Microbial …, 2021 - search.ebscohost.com
The removal of unwanted genetic material is a key aspect in many synthetic biology efforts
and often requires preliminary knowledge of which genomic regions are dispensable …

LoxTnSeq: Random Transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions

D Shaw, S Miravet-Verde, C Pinero, L Serrano… - bioRxiv, 2020 - biorxiv.org
The removal of unwanted genetic material is a key aspect in many synthetic biology efforts,
and often requires preliminary knowledge of which genomic regions are dispensable …

LoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions

D Shaw, S Miravet‐Verde… - Microbial …, 2021 - search.proquest.com
sequential genome reduction has been used as an engineering tool in Bacillus subtilis,
creating a mutant strain with a boosted capacity for production of heterologous proteins by …

[PDF][PDF] LoxTnSeq: Random Transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions

D Shaw, S Miravet-Verde, C Pinero, L Serrano… - scholar.archive.org
One of the core principles behind synthetic biology is the rational engineering of an
organism towards a specific application (D'Halluin and Ruiter, 2013; Esvelt and Wang, 2013; …

LoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions

D Shaw, S Miravet‐Verde… - Microbial …, 2021 - Wiley Online Library
The removal of unwanted genetic material is a key aspect in many synthetic biology efforts
and often requires preliminary knowledge of which genomic regions are dispensable …

LoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions

D Shaw, S Miravet Verde… - … . 2021; 14 (6): 2403 …, 2021 - repositori.upf.edu
The removal of unwanted genetic material is a key aspect in many synthetic biology efforts
and often requires preliminary knowledge of which genomic regions are dispensable …

LoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions.

D Shaw, S Miravet-Verde, C Piñero-Lambea… - Microbial …, 2020 - europepmc.org
We utilised a novel genome deletion strategy involving the Cre/Lox system, coupled with
random transposon mutagenesis, in the genome reduced bacterium Mycoplasma …

LoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions

D Shaw, S Miravet-Verde… - Microbial …, 2021 - research-collection.ethz.ch
The removal of unwanted genetic material is a key aspect in many synthetic biology efforts
and often requires preliminary knowledge of which genomic regions are dispensable …

LoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions

D Shaw, S Miravet-Verde, C Piñero Lambea… - Microbial …, 2021 - repositori.uic.es
The removal of unwanted genetic material is a key aspect in many synthetic biology efforts
and often requires preliminary knowledge of which genomic regions are dispensable …