16S rRNA gene analysis with QIIME2

M Hall, RG Beiko - Microbiome analysis: methods and protocols, 2018 - Springer
QIIME2 provides a software environment, data standards, and tool wrappers that allow for
… for microbial community analysis. We describe a typical analysis pipeline using QIIME2, and …

Ultrafast and accurate 16S rRNA microbial community analysis using Kraken 2

J Lu, SL Salzberg - Microbiome, 2020 - Springer
… , using both the Greengenes and SILVA 16S rRNA databases. We also show … QIIME 2.
Because we only tested the most recent version of each tool, we will henceforth refer to QIIME 2

Taxonomic annotation of 16S rRNA sequences of pig intestinal samples using MG-RAST and QIIME2 generated different microbiota compositions

J Lima, T Manning, KM Rutherford, ET Baima… - Journal of …, 2021 - Elsevier
QIIME2 as stand-alone tools from start (16S rRNA reads obtained from Illumina in fastQ files)
to finish. For both tools, the SILVA reference database (SSU, release 132) was used in the …

q2-metnet: QIIME2 package to analyse 16S rRNA data via high-quality metabolic reconstructions of the human gut microbiota

F Balzerani, T Blasco, S Pérez-Burillo… - …, 2024 - academic.oup.com
… Here, we present q2-metnet, a QIIME2 plugin that enables the contextualization of 16S
rRNA gene sequencing data into AGORA and AGREDA. In particular, based on relative …

A step-by-step procedure for analysing the 16S rRNA-based microbiome diversity using QIIME 2 and comprehensive PICRUSt2 illustration for functional prediction

A Srivastava, Y Akhter, D Verma - Archives of Microbiology, 2024 - Springer
… with the steps of computational analysis for processing 16S rRNA-based sequences. Here,
… using the QIIME 2 platform along with their analyses, which include installing QIIME 2, …

QIIME 2 enables comprehensive end‐to‐end analysis of diverse microbiome data and comparative studies with publicly available data

M Estaki, L Jiang, NA Bokulich… - Current protocols in …, 2020 - Wiley Online Library
… DNA sequence data into QIIME 2 and creating a visual summary16S rRNA gene) into
QIIME 2. All data used and generated by QIIME 2, with the exception of metadata, exist as QIIME 2

Instruction of microbiome taxonomic profiling based on 16S rRNA sequencing

H Kim, S Kim, S Jung - Journal of Microbiology, 2020 - Springer
… of computational analysis of 16S rRNA sequencing data to … software tools of
microbiome analysis. This instruction may be … original figures in QIIME2 documents [https://docs.qiime2.org/…

16S rRNA amplicon sequencing for metagenomics

H Christensen, AJ Andersson, SL Jørgensen… - … to bioinformatics in …, 2018 - Springer
… major pipelines mothur and QIIME 2. We have … QIIME 2 in more detail in ► Activity 8.10.1.
You will be able to get a good start with the analysis of 16S rRNA amplicon data with QIIME 2. …

Amplicon sequence variant-based oral microbiome analysis using QIIME 2

H Maruyama, A Masago, T Nambu… - Journal of Osaka …, 2020 - jstage.jst.go.jp
… of 16S rRNA genes. Recent amplicon sequence variant (ASV)-based analyses of 16S rRNA
genes … Here, we conducted ASV-based analysis of oral microbiome samples using QIIME 2. …

Impact of DNA sequencing and analysis methods on 16S rRNA gene bacterial community analysis of dairy products

Z Xue, ME Kable, ML Marco - Msphere, 2018 - Am Soc Microbiol
… being wrapped in the QIIME 2 platform, we agree with the QIIME 2 developers that new
sequencing results should be analyzed using QIIME 2 with a standardized analysis pipeline (eg, …