2b-RAD: a simple and flexible method for genome-wide genotyping
We describe 2b-RAD, a streamlined restriction site–associated DNA (RAD) genotyping
method based on sequencing the uniform fragments produced by type IIB restriction
endonucleases. Well-studied accessions of Arabidopsis thaliana were genotyped to validate
the method's accuracy and to demonstrate fine-tuning of marker density as needed. The
simplicity of the 2b-RAD protocol makes it particularly suitable for high-throughput
genotyping as required for linkage mapping and profiling genetic variation in natural …
method based on sequencing the uniform fragments produced by type IIB restriction
endonucleases. Well-studied accessions of Arabidopsis thaliana were genotyped to validate
the method's accuracy and to demonstrate fine-tuning of marker density as needed. The
simplicity of the 2b-RAD protocol makes it particularly suitable for high-throughput
genotyping as required for linkage mapping and profiling genetic variation in natural …
Abstract
We describe 2b-RAD, a streamlined restriction site–associated DNA (RAD) genotyping method based on sequencing the uniform fragments produced by type IIB restriction endonucleases. Well-studied accessions of Arabidopsis thaliana were genotyped to validate the method's accuracy and to demonstrate fine-tuning of marker density as needed. The simplicity of the 2b-RAD protocol makes it particularly suitable for high-throughput genotyping as required for linkage mapping and profiling genetic variation in natural populations.
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