… coli strains and plasmid DNAs used in this study are noted in Table 1. Strains were constructed
using P1vir-mediated generalized transduction [50], λRed-mediated recombination [51], …

… form of clamp rather than the homodimeric mutant [6,49]. The dnaN plasmid shuffle assay
described in this report will not only circumvent this issue, but also provides a rapid …

… form of clamp rather than the homodimeric mutant [6,49]. The dnaN plasmid shuffle assay
described in this report will not only circumvent this issue, but also provides a rapid …

… Bacterial strains and plasmid DNAs used in this study are … shuffle assay to measure the
respective abilities of mutant β … double mutant strains expressing the mutant dnaN alleles …

… mutant β clamp … plasmid pJD100 that expresses physiological levels of dnaN + .19, 28 We
then asked whether viability of this strain was dependent on the plasmid-expressed β + clamp …

… altered in the dnaN E202K mutant compared with a dnaN + strain. For … , Δhda strains rapidly
acquire suppressor mutations that both … Using these plasmid-bearing strains, we previously …

… the mutant dnaN159 strain, which expresses the ß159 clamp … and dnaN mutants bearing
the G174A mutation demonstrate … Using a plasmid shuffle assay as illustrated in Figure 2.5, I …

… level of the β clamp impedes Escherichia coli growth by … a low copy number plasmid using
a plasmid shuffle assay (32… in vivo, we compared this mutant to the isogenic dnaN + strain by …

… a mild mutator phenotype in Escherichia coli, but completely … β (VB100) and cysteine-modified
β (VB101) shuffle strains. … and β-clamp, as well as the double mutant, conferred mutation …

… DnaA with the β‐subunit sliding clamp to promote hydrolysis … mutation was transferred by
P1 phage‐mediated transduction into a strain with or without the hda‐complementing plasmid …