This research was carried out to determine the prevalence state of Hepatitis B infection and predisposing factors among blood donors in Dutse and Gumel General Hospitals, Jigawa State, Nigeria. Five hundred and forty-six (546) blood samples were aseptically collected from consenting donors at two hospitals. Two hundred and seventy-three (273) samples were screened from each General Hospital for Hepatitis B Surface Antigen (HBsAg) using third generation enzyme immunoassay HBsAg kits. Out of 273 samples from Dutse General Hospital, 260 were males, and 13 were females while in Gumel General Hospital, 264 were males, and 9 were females. The prevalence rates in the two General Hospitals were 36 and 27 respectively. The peak age specific HBsAg sero-positivity occurred in the aged range of 31-40yrs and 21-30yrs for Dutse and Gumel General Hospitals respectively. Commercial donors constitute highest percentage of blood donors in the two Hospitals; 25% and 20% respectively. The finding indicates that there is no significant difference between gender and the occurrence of the infection among blood donors attending two General Hospitals p > 0.05. The result confirmed that there is no significant difference between the two hospitals based on the Hepatitis B infection at p > 0.05. Among the predisposing factors analysed statistically, the result shows that there is significant difference p < 0.05 between the level of education, occupational status, donors with history of Alcohol consumption, and the occurrence of Hepatitis B surface Antigen in both hospitals but there is no significant difference p > 0.05 between the Hepatitis B Virus (HBV) vaccination status and the occurrence of HBsAg among donors in both hospitals, so it is important to make thorough investigation among blood donors before transfusion of blood, because transfusion of blood saves life, but recipients are at high risk of contracting Hepatitis B virus infections through transfusion of infected blood and blood products. Mass screening, immunization against the virus, and public health education to enlighten the populace about infection and the routes of transmission are recommended.