A Wor1-Like Transcription Factor Is Essential for Virulence of Cryptococcus neoformans

HC Paes, LS Derengowski, LDF Peconick… - Frontiers in cellular …, 2018 - frontiersin.org
HC Paes, LS Derengowski, LDF Peconick, P Albuquerque, GJ Pappas Jr, AM Nicola
Frontiers in cellular and infection microbiology, 2018frontiersin.org
Gti1/Pac2 transcription factors occur exclusively in fungi and their roles vary according to
species, including regulating morphological transition and virulence, mating and secondary
metabolism. Many of these functions are important for fungal pathogenesis. We therefore
hypothesized that one of the two proteins of this family in Cryptococcus neoformans, a major
pathogen of humans, would also control virulence-associated cellular processes.
Elimination of this protein in C. neoformans results in reduced polysaccharide capsule …
Gti1/Pac2 transcription factors occur exclusively in fungi and their roles vary according to species, including regulating morphological transition and virulence, mating and secondary metabolism. Many of these functions are important for fungal pathogenesis. We therefore hypothesized that one of the two proteins of this family in Cryptococcus neoformans, a major pathogen of humans, would also control virulence-associated cellular processes. Elimination of this protein in C. neoformans results in reduced polysaccharide capsule expression and defective cytokinesis and growth at 37°C. The mutant loses virulence in a mouse model of cryptococcal infection and retains only partial virulence in the Galleria mellonella alternative model at 30°C. We performed RNA-Seq experiments on the mutant and found abolished transcription of genes that, in combination, are known to account for all the observed phenotypes. The protein has been named Required for cytokinesis and virulence 1 (Rcv1).
Frontiers
以上显示的是最相近的搜索结果。 查看全部搜索结果