Objective: To analyze the use of the Institutional Analysis theoretical-methodological framework and of the central concept of institution in its three moments - instituted, instituting and institutionalizing in health studies. Method: Integrative review conducted in databases of PubMed/Medline, LILACS, Scopus, and CINAHL including original research articles published between 2012 and 2017. Results: Analysis of 26 publications. A plurality of study objects was found, and the most common were related to collective health, namely: mental health, violence, family-team relationship, family health, primary care, health of women, children and adolescents, oral health, continuing education, micropolitics of health work, health surveillance, HIV, law, pedagogical practices, school education and Autism. Nursing and psychology were the areas that most opted for Institutional Analysis. The polysemy of the institution concept was confirmed in the findings, indicating the richness of modes and uses of the concepts. Conclusion: Institutional Analysis presents tool-concepts to perform analyzes of health practices consistent with the Unified Health System.