Titanium alloys have been used extensively in aerospace and medical applications due to their exceptional strength to weight ratio, biocompatibility, and corrosion resistance. While these alloys are known to be difficult to machine, they are typically weldable. Therefore, various titanium-based alloys have been recently considered for production via additive manufacturing technology. Additively manufactured titanium alloys are used to produce a wide range of high-performance components, which are often under cyclic or periodic loading. While the most commonly used titanium alloy (i.e. Ti-6Al-4V) has been extensively characterized, there is a gap in the literature with regards to the fatigue performance of many other titanium alloys considered for additive manufacturing. This study aims at assessing the microstructural, mechanical, and fatigue performance of two additively manufactured titanium-based alloys, Ti-5Al-5V-5Mo-3Cr and Ti-5Al-5Mo-5V-1Cr-1Fe, and comparing the results with the ones for the well-studied Ti-6Al-4V. An EOS M290 laser beam powder bed fusion (LB-PBF) additive manufacturing machine is used to fabricate specimens from various titanium alloys for this study. Specimens are characterized and compared side by side for their porosity, microstructure, tensile, and fatigue behavior.