A disposable electrochemical biosensor for L-DOPA determination in undiluted human serum

B Brunetti, G Valdés-Ramírez, I Litvan… - Electrochemistry …, 2014 - Elsevier
Electrochemistry communications, 2014Elsevier
A disposable electrochemical biosensor for the determination of l-DOPA in undiluted human
serum is described. It is based on the use of a carbon nanotubes-modified screen printed
electrode covered with an electropolymerized poly (thionine) film and an outer cross-linked
layer containing tyrosinase. Further coverage of the sensor with a Nafion layer allows its use
directly in human serum, obviating the need for sample pretreatment. The sensor exhibited a
linear behavior in the 0.8–22 μM concentration range and offers promise for decentralized …
Abstract
A disposable electrochemical biosensor for the determination of l-DOPA in undiluted human serum is described. It is based on the use of a carbon nanotubes-modified screen printed electrode covered with an electropolymerized poly(thionine) film and an outer cross-linked layer containing tyrosinase. Further coverage of the sensor with a Nafion layer allows its use directly in human serum, obviating the need for sample pretreatment. The sensor exhibited a linear behavior in the 0.8–22 μM concentration range and offers promise for decentralized clinical testing of l-DOPA.
Elsevier
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