A genome wide CRISPR/Cas9 screen identifies calreticulin as a selective repressor of ATF6α
Abstract Activating transcription factor 6 (ATF6) is one of three endoplasmic reticulum (ER)
transmembrane stress sensors that mediate the unfolded protein response (UPR). Despite
its crucial role in long-term ER stress adaptation, regulation of ATF6 alpha (α) signalling
remains poorly understood, possibly because its activation involves ER-to-Golgi and nuclear
trafficking. Here, we generated an ATF6α/IRE1 dual UPR reporter CHO-K1 cell line and
performed an unbiased genome-wide CRISPR/Cas9 mutagenesis screen to systematically …
transmembrane stress sensors that mediate the unfolded protein response (UPR). Despite
its crucial role in long-term ER stress adaptation, regulation of ATF6 alpha (α) signalling
remains poorly understood, possibly because its activation involves ER-to-Golgi and nuclear
trafficking. Here, we generated an ATF6α/IRE1 dual UPR reporter CHO-K1 cell line and
performed an unbiased genome-wide CRISPR/Cas9 mutagenesis screen to systematically …
[HTML][HTML] A genome wide CRISPR/Cas9 screen identifies calreticulin as a selective repressor of ATF6⍺
Activating transcription factor 6 (ATF6) is one of three endoplasmic reticulum (ER)
transmembrane stress sensors that mediate the unfolded protein response (UPR). Despite
its crucial role in long-term ER stress adaptation, regulation of ATF6 alpha (⍺) signalling
remains poorly understood, possibly because its activation involves ER-to-Golgi and nuclear
trafficking. Here, we generated an ATF6⍺/IRE1 dual UPR reporter CHO-K1 cell line and
performed an unbiased genome-wide CRISPR/Cas9 mutagenesis screen to systematically …
transmembrane stress sensors that mediate the unfolded protein response (UPR). Despite
its crucial role in long-term ER stress adaptation, regulation of ATF6 alpha (⍺) signalling
remains poorly understood, possibly because its activation involves ER-to-Golgi and nuclear
trafficking. Here, we generated an ATF6⍺/IRE1 dual UPR reporter CHO-K1 cell line and
performed an unbiased genome-wide CRISPR/Cas9 mutagenesis screen to systematically …
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