A high-resolution digital DNA melting platform for robust sequence profiling and enhanced genotype discrimination
M Sinha, H Mack, TP Coleman… - SLAS TECHNOLOGY …, 2018 - journals.sagepub.com
DNA melting analysis provides a rapid method for genotyping a target amplicon directly after
PCR amplification. To transform melt genotyping into a broad-based profiling approach for
heterogeneous samples, we previously proposed the integration of universal PCR and melt
analysis with digital PCR. Here, we advanced this concept by developing a high-resolution
digital melt platform with precise thermal control to accomplish reliable, high-throughput heat
ramping of microfluidic chip digital PCR reactions. Using synthetic DNA oligos with defined …
PCR amplification. To transform melt genotyping into a broad-based profiling approach for
heterogeneous samples, we previously proposed the integration of universal PCR and melt
analysis with digital PCR. Here, we advanced this concept by developing a high-resolution
digital melt platform with precise thermal control to accomplish reliable, high-throughput heat
ramping of microfluidic chip digital PCR reactions. Using synthetic DNA oligos with defined …
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