[HTML][HTML] A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay
BL Sievers, T Gelbart, GS Tan - STAR protocols, 2022 - Elsevier
BL Sievers, T Gelbart, GS Tan
STAR protocols, 2022•ElsevierEvaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in
defining correlates of protection. We describe an assay that uses single-cycle vesicular
stomatitis virus (VSV) pseudoviruses linking a fluorophore with a spike (S) from a variant of
concern (VOC). Using two fluorophores linked to two VOC S, respectively, allows us to
determine the neutralization titer against two VOCs in a single run. This is a generalizable
approach that saves time, samples, and run-to-run variability. For complete details on the …
defining correlates of protection. We describe an assay that uses single-cycle vesicular
stomatitis virus (VSV) pseudoviruses linking a fluorophore with a spike (S) from a variant of
concern (VOC). Using two fluorophores linked to two VOC S, respectively, allows us to
determine the neutralization titer against two VOCs in a single run. This is a generalizable
approach that saves time, samples, and run-to-run variability. For complete details on the …
Summary
Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with a spike (S) from a variant of concern (VOC). Using two fluorophores linked to two VOC S, respectively, allows us to determine the neutralization titer against two VOCs in a single run. This is a generalizable approach that saves time, samples, and run-to-run variability.
For complete details on the use and execution of this protocol, please refer to Sievers et al. (2022).1
Elsevier
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