Irritable bowel syndrome (IBS) is a digestive tract functional disorder, without anatomical or structural problems, manifesting through stool abnormalities, diarrhea or constipation or both forms alternating between them over time, accompanied by abdominal pain, bloating or flatulence. These symptoms contribute not only to poor digestive absorption, disturbed intestinal motility, but can alter gut microbiota, induce intestinal inflammation through immunity mediators, and negatively impact the psychology of the person. The literature concerning animal models of IBS encompasses numerous studies based on unpredictabile chronic mild stressors applied on rodents, rats. Generally, the stress paradigms consist in exposing the animals to a series of randomly alternating micro-stressors. A key aspect when applying the low intensity stress factors is to maximize their degree of unpredictability by a randomized order, as well as the period of exposure which may last from 2 to 16 weeks. Considering the time and resource-demanding procedures for establishing such IBS stress models, we were interested in developing a reliable and effective model that would result also in the emotional depressive changes accompanying the gastrointestinal disturbances. Thus, our approach was to combine water avoidance stress, an equally reliable acute and chronic stressor, as a constantly repeated factor, with randomized unpredictable mild stressors. Thus, this is a new approach and a new combination that we presumed to be efficient even if applied for a shorter period of one week. Our results are suggesting that the current protocol of 1-week-long multiple heterotypic combination with homotypic chronic stress can be effective to replicate the behavioral manifestations similar to IBS symptoms and the accompanying depression-like features. We would presume this would be more efficient if applied for longer time, although in the present case it did work, even if applied for a shorter period of one week.